HES5 Antibody - BSA Free

Western Blot: HES5 Antibody [NBP2-41305] - Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 ug/ml (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry: HES5 Antibody - BSA Free [NBP2-41305] - Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: HES5 Antibody - BSA Free [NBP2-41305] - Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 ug/mL.Green: HES5 Antibody Blue: DAPI staining

Immunocytochemistry/ Immunofluorescence: HES5 Antibody - BSA Free [NBP2-41305] - Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HES5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HES5 Antibody - BSA Free (NBP2-41305) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-HES5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5. Amino Acid Squence: EKNRLRKPVVEKMRRDRIN
• Specificity: At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HES5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for HES5 Antibody - BSA Free

• BHLHB38
• bHLHb38Class B basic helix-loop-helix protein 38
• hairy and enhancer of split 5 (Drosophila)
• Hairy and enhancer of split 5
• transcription factor HES-5

Background

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HES5 Antibody (NBP2-41305)(1)

Publication using NBP2-41305

• Broner E, Trujillo J, Korzinkin M Et Al. Doublecortin-Like Kinase 1 (DCLK1) Is a Novel NOTCH Pathway Signaling Regulator in Head and Neck Squamous Cell Carcinoma Frontiers in Oncology 2021-07-16 [PMID: 34336664] (WB)

Bioinformatics

• Gene Symbol: HES5