Hepsin Antibody (506220)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 506220
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Hepsin Antibody (506220) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Hepsin
  Ile163-Leu417
  Accession # P05981
• Specificity: Detects human Hepsin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HPN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hepsin Antibody (506220)

• EC 3.4.21
• EC 3.4.21.106
• Hepsin
• HPN
• serine protease hepsin catalytic chain
• serine protease hepsin
• TMPRSS1
• TMPRSS1serine protease hepsin non-catalytic chain
• Transmembrane protease serine 1
• transmembrane protease, serine 1

Background

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6). rhHepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the recombinant human Hepsin.

1. Leytus, S.P. et al. (1988) Biochemistry 27:1067.
2. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
3. Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
4. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
5. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
6. Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hepsin Antibody (MAB4776)(1)

Publication using MAB4776

• Tervonen T, Belitskin D, Pant S, Englund J, Marques E, Ala-Hongisto H, Nevalaita L, Sihto H, Heikkila P, Leidenius M, Hewitson K, Ramachandra M, Moilanen A, Joensuu H, Kovanen P, Poso A, Klefstrom J Deregulated hepsin protease activity confers oncogenicity by concomitantly augmenting HGF/MET signalling and disrupting epithelial cohesion. Oncogene, 2015-07-13;0(0):ePub. 2015-07-13 [PMID: 26165838] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: HPN
• Uniprot:
  • P05981()