Hephaestin Antibody

Immunohistochemistry-Paraffin: Hephaestin Antibody [NBP1-85483] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Hephaestin Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQAGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFLSNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HEPH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Hephaestin Protein (NBP1-85483PEP)

Reactivity Notes

Mouse (87%), Rat (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Hephaestin Antibody

• EC 1.16.3.1
• hephaestin
• KIAA0698CPL

Background

Hephaestin is encoded by this gene is similar to an iron transport protein found in mouse. The mouse protein is similar to ceruloplasmin, a serum multi-copper ferroxidase, and is thought to be a membrane-bound protein responsible for transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system. In mouse, defects in this gene can lead to severe microcytic anemia. Two transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hephaestin Antibody (NBP1-85483)(1)

Publication using NBP1-85483

• Yanase K, Uemura N, Chiba Y et al. Immunoreactivities for hepcidin, ferroportin, and hephaestin in astrocytes and choroid plexus epithelium of human brains Neuropathology 2019-11-22 [PMID: 31755170]

Bioinformatics

• Gene Symbol: HEPH
• Entrez:
  • 9843(Human)
• Uniprot:
  • Q9BQS7(Human)