Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody (738306) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 738306
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody (738306) [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1
  Met59-Asn356
  Accession # Q7LGA3
• Specificity: Detects human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 in ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody (738306) [Alexa Fluor® 532]

• 2OST
• 2-O-sulfotransferase
• EC 2.8.2
• EC 2.8.2.-
• FLJ11317
• heparan sulfate 2-O-sulfotransferase 1
• HS2ST
• HS2ST1
• KIAA0448dJ604K5.2
• MGC131986

Background

Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. Typically, it is covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues are either glucuronic acid or iduronic acid and maybe sulfated at the 2-O position by heparan sulfate 2‑O sulfotransferase 1 (HS2ST1) (3, 4). HS2ST1 physically interacts in the Golgi apparatus with glucuronyl c5-epimerase (5), which catalyzes the conversion of glucuronic acid to iduronic acid (6). As a consequence, 2-O sulfation predominantly occurs on iduronic acids naturally and overexpression of HS2ST1 alone causes an increase in 2-O sulfation on glucuronic acid (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.