N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) - Azide and BSA Free

Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - NDST1 monoclonal antibody (M01), clone 1G10 Analysis of NDST1 expression in A-549.

Immunohistochemistry-Paraffin: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - Analysis of monoclonal antibody to NDST1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - Western Blot analysis of NDST1 expression in A-549 ( Cat # L025V1 ).

Sandwich ELISA: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - Detection limit for recombinant GST tagged NDST1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NDST1 (NP_001534, 38 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LYGWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALI
• Specificity: NDST1 - N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NDST1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) - Azide and BSA Free

• [Heparan sulfate]-glucosamine N-sulfotransferase 1
• bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
• EC 2.8.2.8
• Glucosaminyl N-deacetylase/N-sulfotransferase 1
• heparan sulfate-N-deacetylase/N-sulfotransferase
• HSNST 1
• HSNST1
• HSST1
• HSSTN-HSST 1
• N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
• NDST1
• NDST-1
• N-heparan sulfate sulfotransferase 1
• NST1

Background

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine(GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backboneto make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a rolein determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence isabsolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands,thereby playing a role in inflammatory response

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (H00003340-M01)(1)

Publication using H00003340-M01

• Baljinnyam E, Iwatsubo K, Kurotani R et al. Epac increases melanoma cell migration by a heparan sulfate-related mechanism. Am J Physiol Cell Physiol 297(4):C802-13. 2009-10-01 [PMID: 19657062]

Bioinformatics

• Gene Symbol: NDST1
• Entrez:
  • 3340(Human)
• OMIM:
  • 600853(Human)
• Uniprot:
  • NP_001534(Human)