Hemopexin Antibody (781941) [Alexa Fluor® 594]

• Applications: WB
• Clone: 781941
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

Hemopexin Antibody (781941) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Hemopexin
  Met1-Gln460
  Accession # Q91X72
• Specificity: Detects mouse Hemopexin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Hemopexin is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hemopexin Antibody (781941) [Alexa Fluor® 594]

• Beta-1B-glycoprotein
• FLJ56652
• Hemopexin
• HPX
• HX

Background

HPX (Hemopexin) is a 60-68 kDa secreted member of the hemopexin family of molecules. Although it is principally expressed by hepatocytes, it is also reportedly secreted by macrophages, neurons, astrocytes, renal mesangial cells and fibroblasts. It is considered an acute phase protein, and demonstrates a number of effects. Principally, it binds free and albumin-bound heme, neutralizing its potential for oxidative damage to cell membranes. Heme is removed from HPX internally following binding of the HPX:heme complex to CD91 on hepatocytes and macrophages. HPX also dampens the proinflammatory response of macrophages to TLR2 and TLR4 activation. And HPX also shows serine protease activity which may impact cell surface proteins and ECM. Mature mouse HPX is a 437 amino acid (aa) glycoprotein (aa 24-460). It contains five consecutive HPX-like domains (aa 56-349) that likely impart binding-partner specificity to HPX. There are multiple potential isoform variants. One shows a two aa substitution for aa 134-460, a second contains a deletion of aa 60-100, a third possesses a 14 aa substitution for aa 235-460, while a fourth shows a two aa substitution for aa 153-460. Mature mouse HPX shares 92% and 77% aa identity with rat and human HPX, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q91X72(Mouse)