Recombinant Human Hemoglobin zeta Protein

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SDS-Page: HBZ Protein [NBP1-44486] - HBZ, 16.7kDa (150aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Hemoglobin zeta Protein Summary

Description
A recombinant protein with a His tag and corresponding to amino acids 1 - 142 of HBZ.

Source: E. coli

Amino Acid Sequence: MSLTKTERTI IVSMWAKIST QADTIGTETL ERLFLSHPQT KTYFPHFDLH PGSAQLRAHG SKVVAAVGDA VKSIDDIGGA LSKLSELHAY ILRVDPVNFK LLSHCLLVTL AARFPADFTA EAHAAWDKFL SVVSSVLTEK YRLEHHHHHH

Preparation
Method
E.coli
Protein/Peptide Type
Recombinant Protein
Gene
HBZ
Purity
>90%, by SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 100mM NaCl, 20% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This is a Human protein

Notes

The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 16.7kDa (150aa), confirmed by MALDI-TOF

Alternate Names for Recombinant Human Hemoglobin zeta Protein

  • HBAZ
  • HBZ
  • HBZ2
  • hemoglobin subunit zeta
  • Hemoglobin zeta chain
  • Hemoglobin zeta
  • hemoglobin, zeta
  • zeta-globin

Background

Hemoglobin subunit zeta (HBZ) is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The HBZ gene includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. Recombinant human HBZ fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol HBZ
Entrez
Uniprot

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