Hemoglobin beta Antibody (1G8) [Janelia Fluor® 525] - Chimeric

• Reactivity: Hu
• Applications: ELISA
• Clone: 1G8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Janelia Fluor 525

Hemoglobin beta Antibody (1G8) [Janelia Fluor® 525] - Chimeric Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The original antibody was generated by immunizing BALB/c mice with synthetic N-terminal glycopeptide (VHLTPEC) and KLH conjugate. (Uniprot# P68871)
• Specificity: This antibody binds human hemoglobin glycated at the N-terminal valine of the hemoglobin beta chain and does not cross react with non-glycated hemoglobin.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HBB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Hemoglobin beta Antibody (1G8) [Janelia Fluor® 525] - Chimeric

• beta globin chain
• beta-globin
• CD113t-C
• HBBB
• HBD
• Hemoglobin beta chain
• hemoglobin subunit beta
• hemoglobin, beta

Background

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HBB