HDAC1 Antibody (CL0510) - Azide and BSA Free

Analysis in human rectum and skeletal muscle tissues using NBP3-44032 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.

Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human rectum shows strong nuclear positivity in glandular cells.

Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Staining of U-251 cells using the Anti-HDAC1 monoclonal antibody) .

Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL0510
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HDAC1 Antibody (CL0510) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HDAC1 Antibody (CL0510) - Azide and BSA Free (NBP2-52937) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q13547, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HDAC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for HDAC1 Antibody (CL0510) - Azide and BSA Free

• EC 3.5.1.98
• GON-10
• HD1
• HD1DKFZp686H12203
• Histone Deacetylase 1
• KDAC1
• reduced potassium dependency, yeast homolog-like 1
• RPD3L1
• RPD3L1RPD3

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC1