HCC1 Antibody - BSA Free

Western Blot: HCC1 Antibody [NB200-333] - Detection of human Caper by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caper antibody NB200-333 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: HCC1 Antibody [NB200-333] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.

Immunoprecipitation: HCC1 Antibody [NB200-333] - Detection of human HCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HCC1 antibody NB200-333 (lot 2) used for IP at 6 ug per reaction. HCC1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated HCC1, NB200-333 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HCC1 Antibody - BSA Free Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residues 325-375 of human Caper, Coactivator of activating protein-1 and estrogen receptors using the SwissProt entry Q14498 (GeneID 9584).
• Predicted Species: Mouse (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM39
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  HCC1 Overexpression Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HCC1 Antibody - BSA Free

• CAPER alpha
• CAPER
• CAPERalpha
• CC1.3
• coactivator of activating protein-1 and estrogen receptors
• DKFZp781C0423
• FLJ44170
• fSAP59
• functional spliceosome-associated protein 59
• HCC1
• HCC1CAPERalpha
• Hepatocellular carcinoma protein 1
• RBM39
• RNA binding motif protein 39
• RNA-binding motif protein 39
• RNA-binding protein 39
• RNA-binding region-containing protein 2
• RNPC2
• RRM) containing 2
• splicing factor CC1.3
• Splicing factor HCC1

Background

Transcriptional coactivators either bridge transcription factors and the components of the basal transcription apparatus and/or remodel the chromatin structures. CAPER (coactivator of activating protein-1 (AP-1) and estrogen receptors (ERs)) may represent a selective transcriptional coactivator molecule that plays a pivotal role for the function of AP-1 and ERs in vivo in conjunction with the general coactivator ASC-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HCC1 Antibody (NB200-333)(7)

Publications using NB200-333

• Huang G, Zhou Z, Wang H et al. CAPER-alpha alternative splicing regulates the expression of vascular endothelial growth factor??? in Ewing sarcoma cells. Cancer 2012-04-01 [PMID: 22009261]
• Dutta J, Fan G, Gelinas C et al. CAPERalpha is a novel Rel-TAD-interacting factor that inhibits lymphocyte transformation by the potent Rel/NF-kappaB oncoprotein v-Rel. J Virol 2008-11-01 [PMID: 18753212]
• Ellis JD, Lleres D, Denegri M et al. Spatial mapping of splicing factor complexes involved in exon and intron definition. J Cell Biol 2008-06-01 [PMID: 18559666]
• Kim K, Choi J, Heo K et al. Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription. J Biol Chem 2008-04-01 [PMID: 18258596]
• Choi J, Kim B, Heo K et al. Purification and characterization of cellular proteins associated with histone H4 tails. J Biol Chem 2007-07-01 [PMID: 17548343]
• Meng Q, Rayala SK, Gururaj AE et al. Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1. Proc Natl Acad Sci U S A 2007-04-01 [PMID: 17389360]
• Jung SY, Malovannaya A, Wei J et al. Proteomic analysis of steady-state nuclear hormone receptor coactivator complexes. Mol Endocrinol 2005-10-01 [PMID: 16051665]

Bioinformatics

• Gene Symbol: RBM39
• Entrez:
  • 9584(Human)
• Uniprot:
  • Q14498(Human)