HCC1 Overexpression Lysate (Native)

Images

 
Western Blot: HCC1 Overexpression Lysate (Adult Normal) [NBL1-15210] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCC1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HCC1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC224584 and based on accession number NM_184234. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens RNA binding motif protein 39 (RBM39), transcript variant 1, mRNA.
Gene
RBM39

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HCC1 Overexpression Lysate (Native)

  • CAPER
  • CC1.3
  • coactivator of activating protein-1 and estrogen receptors
  • DKFZp781C0423
  • FLJ44170
  • fSAP59
  • functional spliceosome-associated protein 59
  • HCC1CAPERalpha
  • Hepatocellular carcinoma protein 1
  • RNA binding motif protein 39
  • RNA-binding motif protein 39
  • RNA-binding protein 39
  • RNA-binding region-containing protein 2
  • RNPC2
  • RRM) containing 2
  • splicing factor CC1.3
  • Splicing factor HCC1

Background

The protein encoded by this gene is an RNA binding protein and possible splicing factor. The encoded protein is found in the nucleus, where it colocalizes with core spliceosomal proteins. Studies of a mouse protein with high sequence similarity to this protein suggest that this protein may act as a transcriptional coactivator for JUN/AP-1 and estrogen receptors. Multiple transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (a), also called CC1.4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB200-335
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB110-61646
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-67861
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89544
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45354
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-92553
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB110-75039
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56530
Species: Hu
Applications: WB
NBP2-48480
Species: Hu
Applications: Flow, IHC-Fr, Flow-CS
NBP2-33397
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
7954-GM/CF
Species: Hu
H00009768-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF143
Species: Hu
Applications: WB, Simple Western, IHC
AF2699
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC

Publications for HCC1 Lysate (NBL1-15210) (0)

There are no publications for HCC1 Lysate (NBL1-15210).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HCC1 Lysate (NBL1-15210) (0)

There are no reviews for HCC1 Lysate (NBL1-15210). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HCC1 Lysate (NBL1-15210) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for HCC1 Lysate (NBL1-15210)

Discover related pathways, diseases and genes to HCC1 Lysate (NBL1-15210). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HCC1 Lysate (NBL1-15210)

Discover more about diseases related to HCC1 Lysate (NBL1-15210).
 

Pathways for HCC1 Lysate (NBL1-15210)

View related products by pathway.

Research Areas for HCC1 Lysate (NBL1-15210)

Find related products by research area.

Blogs on HCC1

There are no specific blogs for HCC1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GM-CSF
7954-GM-010/CF
HCC1 Antibody
NBP1-88200

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HCC1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RBM39