HCC1 Overexpression Lysate


Western Blot: HCC1 Overexpression Lysate (Adult Normal) [NBL1-15210] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCC1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

HCC1 Overexpression Lysate Summary

The lysate was created in HEK293T cells, using plasmid ID RC224584 and based on accession number NM_184234. The protein contains a C-terminal DDK tag.
Homo sapiens RNA binding motif protein 39 (RBM39), transcript variant 1, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HCC1 Overexpression Lysate

  • CC1.3
  • coactivator of activating protein-1 and estrogen receptors
  • DKFZp781C0423
  • FLJ44170
  • fSAP59
  • functional spliceosome-associated protein 59
  • HCC1CAPERalpha
  • Hepatocellular carcinoma protein 1
  • RNA binding motif protein 39
  • RNA-binding motif protein 39
  • RNA-binding protein 39
  • RNA-binding region-containing protein 2
  • RNPC2
  • RRM) containing 2
  • splicing factor CC1.3
  • Splicing factor HCC1


The protein encoded by this gene is an RNA binding protein and possible splicing factor. The encoded protein is found in the nucleus, where it colocalizes with core spliceosomal proteins. Studies of a mouse protein with high sequence similarity to this protein suggest that this protein may act as a transcriptional coactivator for JUN/AP-1 and estrogen receptors. Multiple transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (a), also called CC1.4.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Gene Symbol RBM39