Haptoglobin Antibody (HP/3835) - Azide and BSA Free

Protein Array: Haptoglobin Antibody (HP/3835) - Azide and BSA Free [NBP3-14067] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Haptoglobin Antibody (HP/3835).

Formalin-fixed, paraffin-embedded human liver stained with Haptoglobin Antibody (HP/3835) - Azide and BSA Free at 2ug/ml.

Formalin-fixed, paraffin-embedded human liver stained with Haptoglobin Antibody (HP/3835) - Azide and BSA Free at 2ug/ml.

• Reactivity: Hu
• Applications: IHC, MA
• Clone: HP/3835
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Haptoglobin Antibody (HP/3835) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13939).
  
  Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
  
  Theoretical molecular weight: 15-18 kDa (chain); 45kDa (chain)
• Immunogen: Recombinant fragment (around aa100-250) of human Haptoglobin protein (exact sequence is proprietary) (Uniprot: P00738)
• Localization: Secreted. Selective granular cytoplasmic expression in liver with distinct positivity in plasma and extracellular matrix
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Haptoglobin Antibody (HP/3835) - Azide and BSA Free

• binding peptide
• BP
• haptoglobin alpha(1S)-beta
• haptoglobin alpha(2FS)-beta
• Haptoglobin
• haptoglobin, alpha polypeptide
• haptoglobin, beta polypeptide
• HP
• HP-1
• hp2-alpha
• HP2ALPHA2
• HPA1S
• MGC111141
• zonulin

Background

Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HP