HAP1 Antibody

Western Blot: HAP1 Antibody [NB100-55413] - Staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: HAP1 Antibody [NB100-55413] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HAP1 Antibody Summary

• Immunogen: Peptide with sequence C-RYDFRYSEDREQ corresponding to internal region according to NP_003940.2, NP_817084.1.
• Epitope: RYDFRYSEDREQ
• Specificity: This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human brain (frontal cortex and hippocampus) lysates (calculated MW of 69.6 kDa band according to NP_817084.1). An additional band of unknown identity was also consistently observed at 25 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Human Brain Hippocampus Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HAP1 Antibody

• HAP2huntingtin-associated protein 2
• hHLP1
• HIP5
• HLP1
• HLPHAP-1
• huntingtin-associated protein 1
• Neuroan 1

Background

Huntingtons disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAP1 Antibody (NB100-55413)(1)

Publication using NB100-55413

• Takeshita Y, Fujinaga R, Zhao C et al. Huntingtin-associated protein 1 (HAP1) interacts with androgen receptor (AR) and suppresses SBMA-mutant-AR-induced apoptosis. Hum Mol Genet 2006-08-01 [PMID: 16782802]

Bioinformatics

• Gene Symbol: HAP1
• Entrez:
  • 9001(Human)
• Uniprot:
  • P54257(Human)