HAP1 Antibody

Images

 
Western Blot: HAP1 Antibody [NB100-55413] - Staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: HAP1 Antibody [NB100-55413] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HAP1 Antibody Summary

Immunogen
Peptide with sequence C-RYDFRYSEDREQ corresponding to internal region according to NP_003940.2, NP_817084.1.
Epitope
RYDFRYSEDREQ
Specificity
This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 70 kDa band observed in human brain (frontal cortex and hippocampus) lysates (calculated MW of 69.6 kDa band according to NP_817084.1). An additional band of unknown identity was also consistently observed at 25 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Control
Human Brain Hippocampus Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-55413.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HAP1 Antibody

  • HAP2huntingtin-associated protein 2
  • hHLP1
  • HIP5
  • HLP1
  • HLPHAP-1
  • huntingtin-associated protein 1
  • Neuroan 1

Background

Huntingtons disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, ChIP, KD
NBP2-75531
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-19535
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, ChIP
NBP2-47525
Species: Hu
Applications: WB, IHC, IHC-P
NB100-2593
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NB100-56082
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NBP1-88871
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-81776
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-47123
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-86872
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1892
Species: Hu
Applications: WB, ICC/IF, PEP-ELISA
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-45731
Species: All-NA
Applications: WB, IHC, IHC-P
NBP2-92637
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-77685
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-55413
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for HAP1 Antibody (NB100-55413)(1)

Reviews for HAP1 Antibody (NB100-55413) (0)

There are no reviews for HAP1 Antibody (NB100-55413). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HAP1 Antibody (NB100-55413) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional HAP1 Products

Array NB100-55413

Bioinformatics Tool for HAP1 Antibody (NB100-55413)

Discover related pathways, diseases and genes to HAP1 Antibody (NB100-55413). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HAP1 Antibody (NB100-55413)

Discover more about diseases related to HAP1 Antibody (NB100-55413).
 

Pathways for HAP1 Antibody (NB100-55413)

View related products by pathway.

PTMs for HAP1 Antibody (NB100-55413)

Learn more about PTMs related to HAP1 Antibody (NB100-55413).
 

Research Areas for HAP1 Antibody (NB100-55413)

Find related products by research area.

Blogs on HAP1

There are no specific blogs for HAP1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HAP1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol HAP1
Entrez
Uniprot