Hairless Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

Hairless Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human Hairless
  Met1-Thr277
  Accession # O43593
• Specificity: Detects human Hairless in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hairless Antibody [Alexa Fluor® 750]

• ALUNC
• AU
• AUHSA277165
• FLJ98880
• hairless (mouse) homolog
• hairless homolog (mouse)
• Hairless
• HR
• MUHH
• MUHH1
• protein hairless

Background

Hairless (HR) is a predicted 145‑155 kDa protein that acts as a corepressor for multiple nuclear receptors. Although it was initially associated with atrichia (hairloss) and WISE gene suppression, it is now known to impact multiple genes in different cell types. In general, it binds both HDACs and select unligated nuclear receptors via multiple domains, thereby repressing gene transcription. Human HR is 1189 amino acids (aa) in length. It contains a C6-type zinc finger DNA binding region (aa 600‑625) and a Jmjc domain (aa 946‑1157) that blocks DNA methylation. The 1189 aa isoform is widely expressed, while a shorter isoform showing a deletion of aa 1072‑1126 is an HR isotype associated with hair follicle Keratin-14⁺ keratinocytes. An additional isoform shows a 25 aa substitution for aa 1091‑1189. Over
aa 1‑277, human HR shares 82% aa identity with mouse HR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.