H4 Clustered Histone 1 [ac Lys91] Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys91] Antibody [NBP3-38424] - Immunofluorescence analysis of C6 cells using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37C for 18 hours. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys91] Antibody [NBP3-38424] - Immunofluorescence analysis of HeLa cells using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37C for 18 hours. Blue: DAPI for nuclear staining.

Western Blot: H4 Clustered Histone 1 [ac Lys91] Antibody [NBP3-38424] - Western blot analysis of lysates from NIH/3T3 cells, using H4 Clustered Histone 1 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37C for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 300s.

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys91] Antibody [NBP3-38424] - Immunofluorescence analysis of NIH/3T3 cells using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37C for 18 hours. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

H4 Clustered Histone 1 [ac Lys91] Antibody - BSA Free Summary

• Immunogen: A synthetic acetylated peptide around K91 of human H4 Clustered Histone 1 (NP_003529.1).
  
  Sequence:
  ALKRQ
• Modification: ac Lys91
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H4C1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for H4 Clustered Histone 1 [ac Lys91] Antibody - BSA Free

• H4-16;H4C11;H4C12;H4C13;H4C14;H4C15;H4C2;H4C3;H4C4;H4C5;H4C6;H4C8;H4C9;H4FA
• H4F4
• HIST1H4A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H4C1