H4 Clustered Histone 1 [ac Lys8] Antibody - BSA Free

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys8] Antibody [NBP3-38403] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys8] Antibody [NBP3-38403] - Immunofluorescence analysis of HeLa cells using H4 Clustered Histone 1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys8] Antibody [NBP3-38403] - Immunohistochemistry analysis of paraffin-embedded Human kidney using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys8] Antibody [NBP3-38403] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western Blot: H4 Clustered Histone 1 [ac Lys8] Antibody [NBP3-38403] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37C for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Chromatin Immunoprecipitation: H4 Clustered Histone 1 [ac Lys8] Antibody [NBP3-38403] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using H4 Clustered Histone 1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Chromatin Immunoprecipitation: H4 Clustered Histone 1 [ac Lys8] Antibody [NBP3-38403] - Chromatin immunoprecipitation was performed with 5 ug of cross-linked chromatin from HeLa cells, using 5 ug of H4 Clustered Histone 1 Rabbit pAb and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

H4 Clustered Histone 1 [ac Lys8] Antibody - BSA Free Summary

• Immunogen: A synthetic acetylated peptide around K8 of human H4 Clustered Histone 1 (NP_003529.1).
  
  Sequence:
  GGKGL
• Modification: ac Lys8
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H4C1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation -seq 1:20 - 1:50
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 1:200 - 1:500
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for H4 Clustered Histone 1 [ac Lys8] Antibody - BSA Free

• H4-16;H4C11;H4C12;H4C13;H4C14;H4C15;H4C2;H4C3;H4C4;H4C5;H4C6;H4C8;H4C9;H4FA
• H4F4
• HIST1H4A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H4C1