H4 Clustered Histone 1 [Monomethyl Arg3] Antibody - BSA Free

Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Arg3] Antibody [NBP3-38031] - Immunohistochemistry analysis of paraffin-embedded Rat lung using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Arg3] Antibody [NBP3-38031] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: H4 Clustered Histone 1 [Monomethyl Arg3] Antibody [NBP3-38031] - Immunohistochemistry analysis of paraffin-embedded Rat brain using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [Monomethyl Arg3] Antibody [NBP3-38031] - Immunofluorescence analysis of 293T cells using H4 Clustered Histone 1 Rabbit pAb. Blue: DAPI for nuclear staining.

Western Blot: H4 Clustered Histone 1 [Monomethyl Arg3] Antibody [NBP3-38031] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

H4 Clustered Histone 1 [Monomethyl Arg3] Antibody - BSA Free Summary

• Immunogen: A synthetic monomethylated peptide around R3 of human H4 Clustered Histone 1 (NP_003529.1).
  
  Sequence:
  SGRGK
• Modification: Monomethyl Arg3
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H4C1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for H4 Clustered Histone 1 [Monomethyl Arg3] Antibody - BSA Free

• H4-16;H4C11;H4C12;H4C13;H4C14;H4C15;H4C2;H4C3;H4C4;H4C5;H4C6;H4C8;H4C9;H4FA
• H4F4
• HIST1H4A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H4C1