Guanylyl Cyclase alpha 1 Antibody

Product Discontinued

Guanylyl Cyclase alpha 1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human GCS-alpha-1.
• Specificity: GCS-alpha-1 (D423) detects endogenous levels of GCS-alpha-1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GUCY1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analyzes of GCS-alpha -1 (D423) pAb in extracts from THP1 cells.
  Immunohistochemistry (IHC) analyzes of GCS-alpha -1 (D423) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Guanylyl Cyclase alpha 1 Antibody

• EC 4.6.1.2
• GC-S-alpha-1
• GCS-alpha-1
• GCS-alpha-3
• guanylate cyclase 1, soluble, alpha 3
• guanylate cyclase soluble subunit alpha-3
• Guanylyl Cyclase alpha 1
• GUC1A3
• GUC1A3GUCA3
• GUCSA3
• GUCSA3GC-SA3
• GUCY1A1
• GUCY1A3
• Soluble guanylate cyclase large subunit

Background

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit, such as alpha-1 (GUCY1A3), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Guanylyl Cyclase alpha 1 Antibody (NBP2-13000)(3)

Publications using NBP2-13000

• kessler T, Wobst J, Wolf B et al. Functional Characterization of the GUCY1A3 Coronary Artery Disease Risk Locus. Circulation. 2017-08-01 [PMID: 28487391] (WB, Human)
• Calmasini FB, Silva FH, Alexandre EC et al. Implication of Rho-kinase and soluble guanylyl cyclase enzymes in prostate smooth muscle dysfunction in middle-aged rats. Neurourol. Urodyn. 2016-03-21 [PMID: 26999618] (WB, Rat)
• Ghosh A, Stasch JP, Papapetropoulos A et al. Nitric Oxide and Heat Shock Protein 90 Activate Soluble Guanylate Cyclase by Driving Rapid Change in its Subunit Interactions and Heme Content. J. Biol. Chem. 2014-04-14 [PMID: 24733395] (WB, Rat)

Reviews for Guanylyl Cyclase alpha 1 Antibody (NBP2-13000) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Other.

reviewed by: Verified Customer WB Other 06/20/2014

Summary

• Application: Western Blot
• Sample Tested: PC12 or MEF whole cell lysate
• Species: Other
• Lot: 571201

reviewed by: Verified Customer WB Other 06/17/2014

Summary

• Application: Western Blot
• Sample Tested: PC12 or MEF whole cell lysate
• Species: Other
• Lot: 571201

Bioinformatics

• Gene Symbol: GUCY1A1