GTF2H2 Antibody (PCRP-GTF2H2-1B9) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: Flow, ICC/IF, MA
• Clone: PCRP-GTF2H2-1B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

GTF2H2 Antibody (PCRP-GTF2H2-1B9) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Recombinant full-length human GTF2H2 protein (Uniprot: Q13888)
• Localization: Nucleus
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GTF2H2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Protein Array
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for GTF2H2 Antibody (PCRP-GTF2H2-1B9) [CoraFluor™ 1]

• Basic transcription factor 2 44 kDa subunit
• BTF2 p44
• BTF2
• BTF2P44TFIIH basal transcription factor complex p44 subunit
• General transcription factor IIH polypeptide 2
• general transcription factor IIH subunit 2
• general transcription factor IIH, polypeptide 2 (44kD subunit)
• general transcription factor IIH, polypeptide 2, 44kD subunit
• general transcription factor IIH, polypeptide 2, 44kDa
• MGC102806
• T-BTF2P44
• TFIIH

Background

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2H2