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GSTA4 Antibody

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Western Blot: GSTA4 Antibody [H00002941-D01P] - Analysis of GSTA4 expression in mouse spleen.
Western Blot: GSTA4 Antibody [H00002941-D01P] - Analysis of GSTA4 expression in mouse spleen.
Western Blot: GSTA4 Antibody [H00002941-D01P] - Analysis of GSTA4 expression in Jurkat.
Western Blot: GSTA4 Antibody [H00002941-D01P] - Analysis of GSTA4 expression in human placenta.
Western Blot: GSTA4 Antibody [H00002941-D01P] - Analysis of GSTA4 expression in transfected 293T cell line by GSTA4 polyclonal antibody.Lane 1: GSTA4 transfected lysate(25.70 KDa).Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GSTA4 Antibody Summary

Immunogen
GSTA4 (NP_001503.1, 1 a.a. - 222 a.a.) full-length human protein. MAARPKLHYPNGRGRMESVRWVLAAAGVEFDEEFLETKEQLYKLQDGNHLLFQQVPMVEIDGMKLVQTRSILHYIADKHNLFGKNLKERTLIDMYVEGTLDLLELLIMHPFLKPDDQQKEVVNMAQKAIIRYFPVFEKILRGHGQSFLVGNQLSLADVILLQTILALEEKIPNILSAFPFLQEYTVKLSNIPTIKRFLEPGSKKKPPPDEIYVRTVYNIFRP
Specificity
Reacts with glutathione S-transferase alpha 4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSTA4
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.
Reviewed Applications
Read 1 Review rated 3
using
H00002941-D01P in the following application:
Publications
Read Publication using H00002941-D01P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GSTA4 Antibody

  • DKFZp686D21185
  • EC 2.5.1.18
  • glutathione S-alkyltransferase A4
  • glutathione S-aralkyltransferase A4
  • glutathione S-aryltransferase A4
  • glutathione S-transferase A4
  • Glutathione S-transferase A4-4
  • glutathione S-transferase alpha 4
  • glutathione transferase A4-4
  • GST class-alpha member 4
  • GSTA4-4
  • GTA4
  • S-(hydroxyalkyl)glutathione lyase A4

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining in human small intestine and pancreas tissues using anti-GSTK1 antibody. Corresponding GSTK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
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Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.
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Western Blot: GSTA1 Antibody [NBP1-33586] - Mouse liver lysate/extract.
Immunocytochemistry/Immunofluorescence: GSTA1 Antibody [NBP1-33586] - Analysis of methanol-fixed Hep3B.
Immunohistochemistry-Paraffin: GSTA1 Antibody [NBP1-33586] - Paraffin-embedded PC3 xenograft, using antibody at 1:100 dilution.
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Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSTP1 protein stained by GSTP1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP2-16756] - Analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
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Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the HRP conjugate of NB600-326. Detection of 1.0, 0.3, or 0.1 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).
Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).
Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the Biotin conjugate of NB600-326. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000). Detection: Streptavidin-HRP (0.1 ug/ml) and chemiluminescence with an exposure time of 60 seconds.
(2 Reviews)
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Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 cell using Neutrophil Elastase/ELA2 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.
Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - ELA-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using ELA-2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neutrophils.
(1 Review)
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Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Western blot analysis in mouse liver tissue and rat liver tissue.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody [NBP1-85367] - Staining in human liver and endometrium tissues. Corresponding CYP2E1 RNA-seq data are presented for the same tissues.
Western Blot: Cytochrome P450 2E1 Antibody [NBP1-85367] - Analysis in human liver tissue.
(1 Publication)
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(4 Publications)
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Western Blot: GSTA3 Antibody [NBP1-86140] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
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(1 Publication)
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(1E10.)
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(1 Publication)
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Western Blot: GSTT2 Antibody (3B6) [NBP2-03126] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSTT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTT2.
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Western Blot: GSTM3 Antibody [NBP1-83323] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-256
Immunohistochemistry-Paraffin: GSTM3 Antibody [NBP1-83323] - Staining in human testis and lymph node tissues using anti-GSTM3 antibody. Corresponding GSTM3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GSTM3 Antibody [NBP1-83323] - Staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts and Leydig cells.
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Immunohistochemistry-Paraffin: HspA4 Antibody [NBP1-81696] - Staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
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GSTA4 Antibody
H00002941-D01P
GSTA4 Antibody
Species: Hu, Mu
Applications: WB

Publications for GSTA4 Antibody (H00002941-D01P)(1)


Review for GSTA4 Antibody (H00002941-D01P) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00002941-D01P:
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Western Blot GSTA4 H00002941-D01P
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reviewed by:
Zachary Patinkin
 WB Human 02/18/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman Skeletal Muscle Lysate
SpeciesHuman
Lot12096

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio1:50, 30 min, Protein SImple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Comments

CommentsSimple Western. Non-specific bands above 40 kDa.

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Secondary Antibodies

 

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Western Blot GSTA4 H00002941-D01P
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 Zachary Patinkin
02/18/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol GSTA4
Entrez
Uniprot