GSTA3 Antibody (1F11) - Azide and BSA Free

Western Blot: GSTA3 Antibody (1F11) [H00002940-M01] - GSTA3 monoclonal antibody (M01), clone 1F11 Analysis of GSTA3 expression in HepG2.

Immunohistochemistry-Paraffin: GSTA3 Antibody (1F11) [H00002940-M01] - Analysis of monoclonal antibody to GSTA3 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Sandwich ELISA: GSTA3 Antibody (1F11) [H00002940-M01] - Detection limit for recombinant GST tagged GSTA3 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GSTA3 Antibody (1F11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GSTA3 (AAH20619, 1 a.a. ~ 222 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGKPKLHYFNGRGRMEPIRWLLAAAGVEFEEKFIGSAEDLGKLRNDGSLMFQQVPMVEIDGIKLVQTRAILNYIASKYNLYGKDIKERALIDMYTEGMADLNEMILLLPLCRPEEKDAKIALIKEKTKSRYFPAFEKVLQSHGQDYLVGNKLSRADISLVELLYYVEELDSSLISNFPLLKALKTRISNLPTVKKFLQPGSPRKPPADAKALEEARKIFRF
• Specificity: GSTA3 - glutathione S-transferase A3
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSTA3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GSTA3 Antibody (1F11) - Azide and BSA Free

• EC 2.5.1.18
• glutathione S-alkyltransferase A3
• glutathione S-aralkyltransferase A3
• glutathione S-aryltransferase A3
• glutathione S-transferase A3
• Glutathione S-transferase A3-3
• glutathione S-transferase alpha 3
• GST class-alpha member 3
• GSTA3-3
• GTA3
• MGC22232
• S-(hydroxyalkyl)glutathione lyase A3

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSTA3
• Entrez:
  • 2940(Human)
• OMIM:
  • 605449(Human)
• Uniprot:
  • AAH20619(Human)