GSTA1 Antibody - BSA Free

Western Blot: GSTA1 Antibody [NBP1-32233] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGE GSTA1 antibody dilution: 1:5000

Immunocytochemistry/Immunofluorescence: GSTA1 Antibody [NBP1-32233] - Analysis of methanol-fixed Hep3B, using GSTA1(GTX108012) antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GSTA1 Antibody [NBP1-32233] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Western Blot: GSTA1 Antibody [NBP1-32233] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE GSTA1 antibody, antibody diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GSTA1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein corresponding to human GSTA1
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSTA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Protein A purified

Alternate Names for GSTA1 Antibody - BSA Free

• EC 1.11.1.-
• EC 2.5.1.18
• EC 5.3.3.-
• glutathione S-alkyltransferase A1
• glutathione S-aryltransferase A1
• glutathione S-transferase 2
• glutathione S-transferase A1
• glutathione S-transferase alpha 1
• glutathione S-transferase Ha subunit 1
• GST class-alpha member 1
• GST HA subunit 1
• GST, class alpha, 1
• GST2
• GSTA1
• GSTA1-1
• GST-epsilon
• GTH1
• MGC131939
• S-(hydroxyalkyl)glutathione lyase A1

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GSTA1 Antibody (NBP1-32233)(1)

Publication using NBP1-32233

• Pasello M, Manara MC, Michelacci F et al. Targeting glutathione-S transferase enzymes in musculoskeletal sarcomas: a promising therapeutic strategy Anal Cell Pathol (Amst) 2011-01-01 [PMID: 21673434] (WB, Human)

Bioinformatics

• Gene Symbol: GSTA1
• Entrez:
  • 2938(Human)
• Uniprot:
  • P08263(Human)