GSH2 Antibody

Western Blot: GSH2 Antibody [NBP2-43705] - Analysis of A. 30 ug IMR32 whole cell lysate/extract 12 % SDS-PAGE GSX2 antibody dilution: 1:500.

Immunocytochemistry/Immunofluorescence: GSH2 Antibody [NBP2-43705] - Analysis of Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSX2 protein stained by GSX2 antibody diluted at 1:200. Blue: Hoechst 33342 staining.

Immunoprecipitation: GSH2 Antibody [NBP2-43705] - Analysis of of GSX2 protein from IMR32 whole cell extracts using 5 ug of GSX2 antibody. Western blot analysis was performed using GSX2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Discontinued

GSH2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human GSX2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for GSH2 Antibody

• GS homeobox 2
• GSH2
• Homeobox protein GSH-2

Background

The GSH2 (also known as GSX2) gene codes for a 304 amino acid long, 32 kDA GS homeobox 2 protein that is a transcription factor that binds to the DNA sequence 5'-CNAATTAG-3'. The GSH2 gene has been researched for its role in leukemia as well as neuronitis. Interactions between GSH2 and ABL1 and NKX2-1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GSH2 Antibody (NBP2-43705)(1)

Publication using NBP2-43705

• De Mori R, Severino M, Mancardi MM et al. Agenesis of the putamen and globus pallidus caused by recessive mutations in the homeobox gene GSX2 Brain 2019-08-14 [PMID: 31412107] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: GSX2