Recombinant Human Growth Hormone Animal-Free Protein

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SDS-Page: Recombinant Human Growth Hormone Animal-Free Protein [NBP2-76241] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Human Growth Hormone Animal-Free Protein [NBP2-76241] - Growth Hormone induced proliferation of 9D11 cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Animal Free
Concentration
LYOPH

Order Details

View Available Formulations

Recombinant Human Growth Hormone Animal-Free Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Human Growth Hormone

Source: E. coli

Amino Acid Sequence: MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFEEAYIPKE QKYSFLQNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF

Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
Specificity
Growth Hormone is a monomer, 22.3 kDa (192 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
Bioactive protein but no acceptance criteria limits established
Protein/Peptide Type
Recombinant Protein
Gene
GH1
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Theoretical MW
22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium bicarbonate, pH 8.0
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human Growth Hormone Animal-Free Protein

  • GH1
  • GHIGHD1B
  • GHNGrowth hormone
  • GH-Nsomatotropin
  • growth hormone 1Pituitary growth hormone
  • Growth Hormone
  • hGH-N
  • Somatotropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Growth Hormone Recombinant Protein (NBP2-76241) (0)

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Research Areas for Growth Hormone Recombinant Protein (NBP2-76241)

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Blogs on Growth Hormone.

Growth hormone (GH, somatotropin, hGH, pituitary growth hormone)
GH is a member of the large family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. Additionally, GH is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic...  Read full blog post.

Somatostatin Receptor 2: Treating Patients Who Cannot Stop Growing
Acromegaly is a rare life-shortening disease caused by elevated levels of growth hormone (GH) secreted by a tumor on the pituitary gland. Treatments include somatostatin analogs, which activate somatostatin receptor 2 (SSTR2), reducing GH secretion an...  Read full blog post.

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Bioinformatics

Gene Symbol GH1