Fish Growth Hormone ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 1-5 hours |
Specificity |
This assay has high sensitivity and excellent specificity for detection of fish Growth Hormone. No significant cross-reactivity or interference between fish Growth Hormone and analogues was observed. |
Standard Curve Range |
312.5 pg/mL - 5000 pg/mL |
Sensitivity |
312.5 pg/mL |
Assay Type |
Competitive ELISA |
Inter-Assay |
%CV < 15 |
Intra-Assay |
%CV < 15 |
Spike Recovery |
90 - 98% |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
GH1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Strip (For 96 wells)
- Assay plate
- Conjugate
- HRP-avidin
- Instruction manual
- Standard
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (20 x concentrate)
|
Alternate Names for Fish Growth Hormone ELISA Kit (Colorimetric)
Background
Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Growth Hormone ELISA Kit (NBP3-21153). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.