Mouse Growth Hormone ELISA Kit (Colorimetric)

ELISA: Mouse Growth Hormone ELISA Kit (Colorimetric) [NBP3-08143] - Samples were spiked with high concentrations of Mouse Growth Hormone and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Growth Hormone ELISA Kit (Colorimetric) [NBP3-08143] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Mouse Growth Hormone ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 10.00
• Intra-Assay: %CV < 10.00
• Spike Recovery: 89 - 104%
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GH1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Growth Hormone ELISA Kit (Colorimetric)

• GH1
• GHB5
• GHIGHD1B
• GHN
• GH-N
• GHNGrowth hormone
• GH-Nsomatotropin
• growth hormone 1Pituitary growth hormone
• Growth Hormone
• hGH-N
• IGHD1A
• IGHD2
• Somatotropin

Background

Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Growth Hormone ELISA Kit (NBP3-08143)(1)

Publication using NBP3-08143

• Adil M, Parvathagiri V, Verma A et al. Claudin17 deficient mice display leaky vasculature and organ injury accompanied by oxidative stress and inflammation Research Square 2022-01-24 (ELISA)

FAQs for Growth Hormone ELISA Kit (NBP3-08143). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GH1