GRHL2 Antibody (CL3760) - Azide and BSA Free

Staining of A-431 cells using the Anti-GRHL2 monoclonal antibody) .

Analysis in human prostate and liver tissues using NBP3-44000 antibody. Corresponding GRHL2 RNA-seq data are presented for the same tissues.

Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Staining of human prostate shows strong nuclear positivity in glandular cells.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human skin shows strong nuclear positivity in squamous epithelial cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL3760
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GRHL2 Antibody (CL3760) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q6ISB3, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRHL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for GRHL2 Antibody (CL3760) - Azide and BSA Free

• BOMMGC149294
• Brother of mammalian grainyhead
• deafness, autosomal dominant 28
• DFNA28
• FLJ13782
• grainyhead-like 2 (Drosophila)
• grainyhead-like protein 2 homolog
• TFCP2L3MGC149295
• Transcription factor CP2-like 3FLJ11172

Background

TFCP2L3 is a member of a family of transcription factor genes whose archetype is TFCP2 (MIM 189889), a mammalian ortholog of the Drosophila gene 'grainyhead' (grh).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRHL2