GRB2 Antibody

Images

 
Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western ...read more
Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - (1ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Western Blot: GRB2 Antibody [NB100-866] - Analysis of Jurkat lysate (RIPA buffer, 35ug total protein per lane).
Western Blot: GRB2 Antibody [NB100-866] - (0.03ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Human Thymus (A) and Molt4 (B) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using ...read more
Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - Analysis of human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GRB2 Antibody Summary

Immunogen
Peptide with sequence C-PRNYVTPVNRNV corresponding to C-Terminus according to NP_987102.1.
Localization
Cytoplasmic (probable)
Specificity
This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GRB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry 0.1 - 0.3 ug/ml
  • Immunohistochemistry-Paraffin 0.1-.3 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx. 26 kDa band observed in MOLT4 lysates, of human thymus and of human, mouse, rat, porcine brain and spleen lysates (calculated MW of 25.2 kDa band according to human NP_002077.1 and according to rat NP_110473.2).
IHC-P: Human tonsil stains mantle zone and to a lessser extent germinal centre. Data obtained by previous batch (different goat). Islets of Langerhans of human pancreas is specifically stained.
Positive Control
GRB2 Lysate (NBL1-11326)
Publications
Read Publication using NB100-866.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GRB2 Antibody

  • abundant SRC homology
  • Adapter protein GRB2
  • ASH
  • EGFRBP-GRB2
  • epidermal growth factor receptor-binding protein GRB2
  • GRB2
  • Grb3-3
  • growth factor receptor-bound protein 2
  • growth factor receptor-bound protein 3
  • HT027
  • MST084
  • MSTP084
  • NCKAP2
  • Protein Ash
  • SH2/SH3 adapter GRB2

Background

Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRB2 Antibody (NB100-866)(1)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GRB2
Entrez
Uniprot