GRB2 Antibody

Images

 
Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western ...read more
Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
Western Blot: GRB2 Antibody [NB100-866] - (0.03ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Human Thymus (A) and Molt4 (B) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using ...read more
Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - (1ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GRB2 Antibody Summary

Immunogen
Peptide with sequence C-PRNYVTPVNRNV corresponding to C-Terminus according to NP_987102.1.
Localization
Cytoplasmic (probable)
Specificity
This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GRB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05-0.15 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx. 26 kDa band observed in MOLT4 lysates, of human thymus and of human, mouse, rat, porcine brain and spleen lysates (calculated MW of 25.2 kDa band according to human NP_002077.1 and according to rat NP_110473.2).
IHC-P: Human tonsil stains mantle zone and to a lessser extent germinal centre. Data obtained by previous batch (different goat). Islets of Langerhans of human pancreas is specifically stained.
Control
GRB2 Overexpression Lysate
GRB2 Knockout HeLa Cell Lysate
Publications
Read Publication using NB100-866.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GRB2 Antibody

  • abundant SRC homology
  • Adapter protein GRB2
  • ASH
  • EGFRBP-GRB2
  • epidermal growth factor receptor-binding protein GRB2
  • GRB2
  • Grb3-3
  • growth factor receptor-bound protein 2
  • growth factor receptor-bound protein 3
  • HT027
  • MST084
  • MSTP084
  • NCKAP2
  • Protein Ash
  • SH2/SH3 adapter GRB2

Background

Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00006464-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA
NBP2-50599
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
AF1107
Species: Mu
Applications: WB, IHC
H00003347-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-85466
Species: Hu, Mu, Fe
Applications: WB, IHC, IHC-P
NBP2-67605
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-88220
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP2-98675
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-1598
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
NB100-82001
Species: Hu, Mu, Rt, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-79843
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB110-68800
Species: Hu, Mu
Applications: WB, ICC/IF

Publications for GRB2 Antibody (NB100-866)(1)

Reviews for GRB2 Antibody (NB100-866) (0)

There are no reviews for GRB2 Antibody (NB100-866). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GRB2 Antibody (NB100-866) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional GRB2 Products

Bioinformatics Tool for GRB2 Antibody (NB100-866)

Discover related pathways, diseases and genes to GRB2 Antibody (NB100-866). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GRB2 Antibody (NB100-866)

Discover more about diseases related to GRB2 Antibody (NB100-866).
 

Pathways for GRB2 Antibody (NB100-866)

View related products by pathway.

PTMs for GRB2 Antibody (NB100-866)

Learn more about PTMs related to GRB2 Antibody (NB100-866).
 

Research Areas for GRB2 Antibody (NB100-866)

Find related products by research area.

Blogs on GRB2

There are no specific blogs for GRB2, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GRB2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GRB2
Entrez
Uniprot