GRB2 Antibody [Janelia Fluor® 525]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Janelia Fluor 525

GRB2 Antibody [Janelia Fluor® 525] Summary

• Immunogen: E. coli-derived recombinant human GRB2
  Met1-Val217
  Accession # P62993
• Specificity: Detects human, mouse, and rat GRB2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRB2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for GRB2 Antibody [Janelia Fluor® 525]

• abundant SRC homology
• Adapter protein GRB2
• ASH
• EGFRBP-GRB2
• epidermal growth factor receptor-binding protein GRB2
• GRB2
• Grb3-3
• growth factor receptor-bound protein 2
• growth factor receptor-bound protein 3
• HT027
• MST084
• MSTP084
• NCKAP2
• Protein Ash
• SH2/SH3 adapter GRB2

Background

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRB2