GRB2 Antibody (669604) - (SH2 Domain)

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Human ...read more
GRB2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human GRB2 (SH2 Domain) Monoclonal Antibody (Catalog # MAB38461) at 15 µg/mL overnight at 4 °C. Before incubation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
669604
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GRB2 Antibody (669604) - (SH2 Domain) Summary

Immunogen
E. coli-derived recombinant human GRB2 (SH2 Domain)
Trp60-Glu152
Accession # P62993
Specificity
Detects human GRB2 (SH2 Domain) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GRAP2 (SH2 domain; aa 58-149), rhGRB7 (aa 130-274), or rhGRB14 (SH2 domain; aa 439-535) is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GRB2
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB38461 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GRB2 Antibody (669604) - (SH2 Domain)

  • abundant SRC homology
  • Adapter protein GRB2
  • ASH
  • EGFRBP-GRB2
  • epidermal growth factor receptor-binding protein GRB2
  • GRB2
  • Grb3-3
  • growth factor receptor-bound protein 2
  • growth factor receptor-bound protein 3
  • HT027
  • MST084
  • MSTP084
  • NCKAP2
  • Protein Ash
  • SH2/SH3 adapter GRB2

Background

GRB2 (growth factor receptor-bound 2; also Ash) is a ubiquitously-expressed, 27 kDa member of the GRB2/sem-5 family of adaptor molecules.  It serves as a linker for many intracellular proteins.  Upon BCR ligation, LAB is phosphorylated, binds to GRB2, which subsequently recruits signaling factors.  Upon InsR ligation, GRB2 binds to phosphorylated IRS-1, and serves as a linker to ras-activation.  Human GRB2 is 217 amino acids (aa) in length.  It contains one N-terminal SH3 domain (aa 3-54), a central SH2 domain (aa 60-152), and a C-terminal SH3 domain (aa 160-212).  SH2 domains bind phosphotyrosine motifs; SH3 domains bind proline-rich regions.  There is one splice form that shows a deletion of aa 60-100.  This removes the SH2 domain and initiates apoptosis.  Human GRB2 is over 99% aa identical to mouse and dog GRB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRB2 Antibody (MAB38461)(2)

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Bioinformatics

Gene Symbol GRB2
Entrez
Uniprot