GRB2 Antibody (669604) [Alexa Fluor® 700]

• Applications: WB, IHC
• Clone: 669604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

GRB2 Antibody (669604) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human GRB2 (SH2 Domain)
  Trp60-Glu152
  Accession # P62993
• Specificity: Detects human GRB2 (SH2 Domain) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GRAP2 (SH2 domain; aa 58-149), rhGRB7 (aa 130-274), or rhGRB14 (SH2 domain; aa 439-535) is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

GRB2 (growth factor receptor-bound 2; also Ash) is a ubiquitously-expressed, 27 kDa member of the GRB2/sem-5 family of adaptor molecules.  It serves as a linker for many intracellular proteins.  Upon BCR ligation, LAB is phosphorylated, binds to GRB2, which subsequently recruits signaling factors.  Upon InsR ligation, GRB2 binds to phosphorylated IRS-1, and serves as a linker to ras-activation.  Human GRB2 is 217 amino acids (aa) in length.  It contains one N-terminal SH3 domain (aa 3-54), a central SH2 domain (aa 60-152), and a C-terminal SH3 domain (aa 160-212).  SH2 domains bind phosphotyrosine motifs; SH3 domains bind proline-rich regions.  There is one splice form that shows a deletion of aa 60-100.  This removes the SH2 domain and initiates apoptosis.  Human GRB2 is over 99% aa identical to mouse and dog GRB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.