GRB2 Antibody (11-B11)

Images

 
Western Blot: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of GRB2 on 293 cell lysate using anti-GRB2 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (11-B11) [NBP2-80405] - Staining GRB2 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with ...read more
Immunohistochemistry-Paraffin: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of mouse brain tissue using GRB2 antibody. Primary antibody dilution 1:100. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (11-B11) [NBP2-80405] - Staining GRB2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with ...read more
Immunohistochemistry-Paraffin: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of human colon cancer tissue using GRB2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of rat brain tissue using GRB2 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
11-B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

GRB2 Antibody (11-B11) Summary

Immunogen
Recombinant protein (SwissProt: P62993 Human;SwissProt: Q60631 Mouse;SwissProt: P62994 Rat)
Localization
Endosome, Nucleus, Golgi apparatus.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GRB2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-80405 in the following applications:

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1X TBS(pH7.4), 0.5%BSA, 50%Glycerol
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Protein G purified

Alternate Names for GRB2 Antibody (11-B11)

  • abundant SRC homology
  • Adapter protein GRB2
  • ASH
  • EGFRBP-GRB2
  • epidermal growth factor receptor-binding protein GRB2
  • GRB2
  • Grb3-3
  • growth factor receptor-bound protein 2
  • growth factor receptor-bound protein 3
  • HT027
  • MST084
  • MSTP084
  • NCKAP2
  • Protein Ash
  • SH2/SH3 adapter GRB2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRB2 Antibody (NBP2-80405) (0)

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Review for GRB2 Antibody (NBP2-80405) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-80405:
Filter by Applications
IHC-P
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GRB2 NBP2-80405
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 02/23/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested Mouse brain
SpeciesMouse
Lot11-B11

Comments

CommentsPrimary antibody dilution was 1:100

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRB2 Antibody (NBP2-80405) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GRB2 Antibody (NBP2-80405)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
02/23/2022
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol GRB2