GRB2 Antibody (11-B11)

Western Blot: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of GRB2 on 293 cell lysate using anti-GRB2 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: GRB2 Antibody (11-B11) [NBP2-80405] - Staining GRB2 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of mouse brain tissue using GRB2 antibody. Primary antibody dilution 1:100. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: GRB2 Antibody (11-B11) [NBP2-80405] - Staining GRB2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of human colon cancer tissue using GRB2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of rat brain tissue using GRB2 antibody. Counter stained with hematoxylin.

Product Discontinued

GRB2 Antibody (11-B11) Summary

• Immunogen: Recombinant protein within Human GRB2 aa 21-217 / 217. (SwissProt: P62993 Human; SwissProt: Q60631 Mouse; SwissProt: P62994 Rat)
• Localization: Endosome, Nucleus, Golgi apparatus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRB2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for GRB2 Antibody (11-B11)

• abundant SRC homology
• Adapter protein GRB2
• ASH
• EGFRBP-GRB2
• epidermal growth factor receptor-binding protein GRB2
• GRB2
• Grb3-3
• growth factor receptor-bound protein 2
• growth factor receptor-bound protein 3
• HT027
• MST084
• MSTP084
• NCKAP2
• Protein Ash
• SH2/SH3 adapter GRB2

Background

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for GRB2 Antibody (NBP2-80405) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Samuel Jones IHC-P Mouse 02/23/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: 11-B11

Comments

• Comments: Primary antibody dilution was 1:100

Bioinformatics

• Gene Symbol: GRB2