Human Granzyme B ELISA Kit (Colorimetric)

Images

 
ELISA: Granzyme B ELISA Kit [NBP1-83736] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
0.66 - 480 pg/mL
Sensitivity
0.2 pg/mL

Order Details

Human Granzyme B ELISA Kit (Colorimetric) Summary

Specificity
The interference of other proteinases was evaluated by spiking these enzymes into a human Granzyme B positive serum. There was no crossreactivity detected, namely not with Proteinase 2 (PR3), Tryptase, Cathepsin G (Cath G), Granzyme A, Human Neutrophil Elastase (HNE), Trypsin, Chymotrypsin.
Standard Curve Range
0.66 - 480 pg/mL
Sensitivity
0.2 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
10.4%
Intra-Assay
8.5%
Spike Recovery
The amount of endogenous human Granzyme B in unspiked serum was substracted from the spike values. The overall mean recovery was 117%.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GZMB

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Granzyme B
  3. Biotin-Conjugate anti-human Granzyme B monoclonal antibody
  4. Dilution Buffer Concentrate 5x
  5. Human Granzyme B Standard lyophilized, 960 pg/mL upon reconstitution
  6. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  7. Streptavidin-HRP
  8. Substrate Solution (tetramethyl-benzidine)
  9. Stop Solution (1M Phosphoric acid)

Alternate Names for Human Granzyme B ELISA Kit (Colorimetric)

  • CSPB
  • CTLA-1
  • CTSGL1
  • Fragmentin-2
  • Granzyme B
  • Granzyme-2
  • GRB
  • GrzB
  • GZMB
  • HLP
  • SECT

Background

Granzymes are exogenous serine proteinases (enzymes) that are released from cytoplasmic granules of cytotoxic lymphocytes (CTLs) and NK cells. The name "granzymes" is derived from: granules + enzymes. These granules contain next to granzymes other proteins including a poreforming protein (Perforin). Upon binding of the CTL to a target cell (by CTL-receptor and antigen-presenting MHC molecules on the target cell) the contents of the granules are released in the intercellular space where after perforine will "perforate" the target cell membrane by forming transmembrane pores. Through these pores the granzymes can now enter the cytosol of the target cell. Granzyme B activates the intracellular cascade of caspases finally resulting in the killing of the target cells. Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders. Not all granzymes enter the target cell, part of them also "leak" in to the peripheral blood and other biological fluids. Detectable amounts of granzymes have been found to circulate in healthy volunteers. These soluble granzymes can be measured by ELISAs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
MAB29051
Species: Hu
Applications: IP, CyTOF-ready, ICC, ICFlow
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NBP2-46132
Species: Hu
Applications: WB, IHC, IHC-P
126-FL/CF
Species: Hu
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP2-15184
Species: Hu, Rt, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NB100-58965
Species: Hu
Applications: WB, PEP-ELISA

Publications for Granzyme B ELISA Kit (NBP1-83736) (0)

There are no publications for Granzyme B ELISA Kit (NBP1-83736).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Granzyme B ELISA Kit (NBP1-83736) (0)

There are no reviews for Granzyme B ELISA Kit (NBP1-83736). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Granzyme B ELISA Kit (NBP1-83736). (Showing 1 - 1 of 1 FAQ).

  1. Will this product work with human plasma samples?
    • This kit has been validated with cell culture supernatant and serum. Plasma (EDTA and Heparin) has been tested once and we were able to detect some low values. However, this matrices has not been fully evaluated and thus, we cannot guarantee that this assay will be compatible with plasma.

Additional Granzyme B Products

Bioinformatics Tool for Granzyme B ELISA Kit (NBP1-83736)

Discover related pathways, diseases and genes to Granzyme B ELISA Kit (NBP1-83736). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Granzyme B ELISA Kit (NBP1-83736)

Discover more about diseases related to Granzyme B ELISA Kit (NBP1-83736).
 

Pathways for Granzyme B ELISA Kit (NBP1-83736)

View related products by pathway.

PTMs for Granzyme B ELISA Kit (NBP1-83736)

Learn more about PTMs related to Granzyme B ELISA Kit (NBP1-83736).
 

Research Areas for Granzyme B ELISA Kit (NBP1-83736)

Find related products by research area.

Blogs on Granzyme B.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9: The Suicidal Cell Whisperer
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. Among the subclass of initiator caspases that include...  Read full blog post.

Caspase 7: The Cell's Suicide Switch
Caspase 7 (also known as CASP7, Mch3, ICE-LAP3, CMH-1) is a member of caspase family of cysteine proteases. It is an apoptosis-related cystein peptidase encoded by the CASP7 gene in humans. CASP7 homologous sequences have been identified in nearly all...  Read full blog post.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF

Contact Information

Product PDFs

Review this Product

Be the first to review our Human Granzyme B ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol GZMB
Entrez