Human Granzyme B ELISA Kit (Colorimetric) Summary
The interference of other proteinases was evaluated by spiking these enzymes into a human Granzyme B positive serum. There was no crossreactivity detected, namely not with Proteinase 2 (PR3), Tryptase, Cathepsin G (Cath G), Granzyme A, Human Neutrophil Elastase (HNE), Trypsin, Chymotrypsin.
|Standard Curve Range
0.66 - 480 pg/mL
The amount of endogenous human Granzyme B in unspiked serum was substracted from the spike values.
The overall mean recovery was 117%.
ELISA Kit (Colorimetric)
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Granzyme B
- Biotin-Conjugate anti-human Granzyme B monoclonal antibody
- Dilution Buffer Concentrate 5x
- Human Granzyme B Standard lyophilized, 960 pg/mL upon reconstitution
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
- Stop Solution (1M Phosphoric acid)
Alternate Names for Human Granzyme B ELISA Kit (Colorimetric)
- Granzyme B
Granzymes are exogenous serine proteinases (enzymes) that are released from cytoplasmic granules of cytotoxic lymphocytes (CTLs) and NK cells. The name "granzymes" is derived from: granules + enzymes. These granules contain next to granzymes other proteins including a poreforming protein (Perforin). Upon binding of the CTL to a target cell (by CTL-receptor and antigen-presenting MHC molecules on the target cell) the contents of the granules are released in the intercellular space where after perforine will "perforate" the target cell membrane by forming transmembrane pores. Through these pores the granzymes can now enter the cytosol of the target cell. Granzyme B activates the intracellular cascade of caspases finally resulting in the killing of the target cells. Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders. Not all granzymes enter the target cell, part of them also "leak" in to the peripheral blood and other biological fluids. Detectable amounts of granzymes have been found to circulate in healthy volunteers. These soluble granzymes can be measured by ELISAs.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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Product General Protocols
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FAQs for Granzyme B ELISA Kit (NBP1-83736). (Showing 1 - 1 of 1 FAQ).
Will this product work with human plasma samples?
- This kit has been validated with cell culture supernatant and serum. Plasma (EDTA and Heparin) has been tested once and we were able to detect some low values. However, this matrices has not been fully evaluated and thus, we cannot guarantee that this assay will be compatible with plasma.
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