Granzyme B Antibody (351927) [Phycoerythrin]

NK-92 human natural killer lymphoma cell line was stained with Mouse Anti-Human Granzyme B PE-conjugated Monoclonal Antibody (Catalog # IC2906P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 351927
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Granzyme B Antibody (351927) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Granzyme B
  Gly19-Tyr247
  Accession # P10144
• Specificity: Detects human Granzyme B in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Granzyme A, rhGranzyme H, recombinant mouse (rm) Granzyme B, rmGranzyme C, rmGranzyme D, or rmGranzyme G.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GZMB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme B Antibody (351927) [Phycoerythrin]

• C11
• CCPI
• CGL1
• CGL-1
• CSPB
• CSP-B
• CTLA1
• CTLA-1
• CTSGL1
• Fragmentin-2
• Granzyme B
• Granzyme-2
• GranzymeB
• GRB
• GrzB
• GZMB
• HLP
• SECT

Background

Granzyme B is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of Cytotoxic T Lymphocytes (CTL) and Natural Killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various Caspases and cleaves proteins such as Aggrecan (3). Human Granzyme B is synthesized as a 247 amino acid (aa) precursor with a signal peptide (aa 1-18), a pro peptide (aa 19-20), and a mature chain (aa 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of aa 19-247 was expressed and purified. After being activated by active Cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).

1. Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549 - 1552.
4. Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
5. Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
6. Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Granzyme B Antibody (IC2906P)(5)

Publications using IC2906P

• HJ Park, MS Shin, M Kim, JB Bilsborrow, S Mohanty, RR Montgomery, AC Shaw, S You, I Kang Transcriptomic analysis of human IL-7 receptor alpha low and high effector memory CD8+ T cells reveals an age-associated signature linked to influenza vaccine response in older adults Aging Cell, 2019-05-01;0(0):e12960. 2019-05-01 [PMID: 31044512] (Flow Cytometry, Human)
• T Masu, M Atsukawa, K Nakatsuka, M Shimizu, D Miura, T Arai, H Harimoto, C Kondo, K Kaneko, S Futagami, C Kawamoto, H Takahashi, K Iwakiri Anti-CD137 monoclonal antibody enhances trastuzumab-induced, natural killer cell-mediated cytotoxicity against pancreatic cancer cell lines with low human epidermal growth factor-like receptor 2 expression PLoS ONE, 2018-12-31;13(12):e0200664. 2018-12-31 [PMID: 30596643] (Flow Cytometry, Human)
• V Giacomet, M Masetti, P Nannini, F Forlanini, M Clerici, GV Zuccotti, D Trabattoni Humoral and cell-mediated immune responses after a booster dose of HBV vaccine in HIV-infected children, adolescents and young adults PLoS ONE, 2018-02-14;13(2):e0192638. 2018-02-14 [PMID: 29444185] (Flow Cytometry, Human)
• Dorneles E, Lima G, Teixeira-Carvalho A, Araujo M, Martins-Filho O, Sriranganathan N, Al Qublan H, Heinemann M, Lage A Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51. PLoS ONE, 2015-09-09;10(9):e0136696. 2015-09-09 [PMID: 26352261] (Flow Cytometry, Bovine)
• Peguillet I, Milder M, Louis D, Vincent-Salomon A, Dorval T, Piperno-Neumann S, Scholl S, Lantz O High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer. Cancer Res, 2014-02-17;74(8):2204-16. 2014-02-17 [PMID: 24535711] (ICC, Human)

Bioinformatics

• Gene Symbol: GZMB
• Uniprot:
  • P10144()