GR/NR3C1 Antibody

Product Discontinued

GR/NR3C1 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues D(346) Q K P I F N V I P P I P V G S E N W N R C(367) from human GR.
• Localization: Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
• Specificity: Glucocorticoid Receptor
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR3C1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 5 ug/ml
• Application Notes: ChIP usage was reported in scientific literature (PMID: 17116691; 2007) WB: Detects an approx. 97 kDa protein representing GR from rat liver extract. ICC: Staining of GR in HeLa cells results in cytoplasmic staining in the absence of ligand, and nuclear staining after hormone administration.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 16912069). Sheep reactivity reported in scientific literature (PMID: 17699566). Primate reactivity reported in scientific literature (PMID: 20466055). Rabbit reactivity reported in scientific literature (PMID: 14638726).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 50 ul distilled water to desired concetration.

Alternate Names for GR/NR3C1 Antibody

• GCR
• glucocorticoid receptor
• GR
• GRGCCR
• GRLNuclear receptor subfamily 3 group C member 1
• NR3C1
• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GR/NR3C1 Antibody (NB300-610)(1)

Publication using NB300-610

• He Y, Simons SS Jr. STAMP, a novel predicted factor assisting TIF2 actions in glucocorticoid receptor-mediated induction and repression. Mol Cell Biol. 2007-02-01 [PMID: 17116691]

Review for GR/NR3C1 Antibody (NB300-610) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Toryn Poolman WB Human 08/22/2011

Summary

• Application: Western Blot
• Sample Tested: A549, Sample Amount: 35ug
• Species: Human
• Lot: KJ138107

FAQs for GR/NR3C1 Antibody (NB300-610). (Showing 1 - 1 of 1 FAQ).

1. How do I reconstitute this lyophilized antibody? (lot # PI204118)
   • We recommend reconstituting this antibody with 50ul of dH2O. This will bring your product to a concentration of 1.0mg/ml.

Bioinformatics

• Gene Symbol: NR3C1
• Entrez:
  • 2908(Human)
  • 14815(Mouse)
  • 24413(Rat)