gp130/CD130 Antibody

Images

 
Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

gp130/CD130 Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130
Leu24-Glu619 (Glu619Asp)
Accession # P40189
Specificity
Detects human gp130 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant rat gp130, recombinant mouse gp130, recombinant human (rh) IL-1 sRI, rhIL-1 sRII, rhIL-2 sR beta, rhIL-2 sR gamma, rhIL‑4 sR, and rhIL-6 sR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL6ST
Purity
Immunogen affinity purified
Endotoxin Note
<0.70 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 2-10 ug/mL
Control
gp130/CD130 Lysate (NBP2-65330)
Publications
Read Publications using
AF-228-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for gp130/CD130 Antibody

  • CD130 antigen
  • CD130
  • CDw130
  • DKFZp564F053
  • gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
  • gp130
  • IL-6 receptor subunit beta
  • IL-6R subunit beta
  • IL-6RB
  • IL-6R-beta
  • IL6ST
  • interleukin 6 signal transducer (gp130, oncostatin M receptor)
  • interleukin receptor beta chain
  • interleukin-6 receptor subunit beta
  • Interleukin-6 signal transducer
  • Membrane glycoprotein 130
  • membrane glycoprotein gp130
  • Oncostatin-M receptor subunit alpha

Background

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7270-IL/CF
Species: Hu
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
MAB1799
Species: Hu, Mu, Rt
Applications: WB, IP, ICC, ICFlow, KO
8475-OM/CF
Species: Hu
AF-249-NA
Species: Hu
Applications: WB, Neut
557-NT/CF
Species: Rt
418-ML/CF
Species: Mu
NBP1-86729
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB400-141
Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IP
MAB4260
Species: Hu, Mu, Rt
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
NBP2-67429
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for gp130/CD130 Antibody (AF-228-NA)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, IHC Paraffin-embedded, Neut, WB.


Filter By Application
ICC
(1)
IHC Paraffin-embedded
(1)
Neut
(2)
WB
(3)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF-228-NA Applications Species
McFarland B, Hong S, Rajbhandari R, Twitty G, Gray G, Yu H, Benveniste E, Nozell S NF-kappaB-induced IL-6 ensures STAT3 activation and tumor aggressiveness in glioblastoma. PLoS ONE, 2013;8(11):e78728. 2013 [PMID: 24244348] (Neut,, Human) Neut Human
Zhang Z, Lee JC Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer. Nat. Genet., 2012;44(8):852-860. 2012 [PMID: 22751098] (WB,, Human) WB Human
Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A Disturbances in the LIF pathway in the endometrium among women with unexplained infertility. Fertil. Steril., 2009;91(6):2602-10. 2009 [PMID: 18684446] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells. Fertil. Steril., 2006;86(4):1193-209. 2006 [PMID: 16949591] (ICC,, Human) ICC Human
Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression. Cancer Res., 2005;65(19):8896-904. 2005 [PMID: 16204061] (Neut,, Human) Neut Human
He W, Gong K, Smith DK, Ip NY The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett., 2005;579(20):4317-23. 2005 [PMID: 16051226] (WB,, Human) WB Human
Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy. Circulation, 2003;107(6):798-802. 2003 [PMID: 12591746] (WB,, Human) WB Human

Reviews for gp130/CD130 Antibody (AF-228-NA) (0)

There are no reviews for gp130/CD130 Antibody (AF-228-NA). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for gp130/CD130 Antibody (AF-228-NA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for gp130/CD130 Antibody (AF-228-NA)

Discover related pathways, diseases and genes to gp130/CD130 Antibody (AF-228-NA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for gp130/CD130 Antibody (AF-228-NA)

Discover more about diseases related to gp130/CD130 Antibody (AF-228-NA).
 

Pathways for gp130/CD130 Antibody (AF-228-NA)

View related products by pathway.

PTMs for gp130/CD130 Antibody (AF-228-NA)

Learn more about PTMs related to gp130/CD130 Antibody (AF-228-NA).

Blogs on gp130/CD130

There are no specific blogs for gp130/CD130, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our gp130/CD130 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol IL6ST
Uniprot