GOT2 Antibody (8B11) - BSA Free

Western Blot: GOT2 Antibody (8B11) [NBP2-59428] - The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GOT1 Lane 2.: Recombinant Human GOT2

Immunocytochemistry/Immunofluorescence: GOT2 Antibody (8B11) [NBP2-59428] - Analysis of GOT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: GOT2 Antibody (8B11) [NBP2-59428] - Analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Western Blot: GOT2 Antibody (8B11) [NBP2-59428] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysateLane 3.: Balb/3T3 cell lysate

Western Blot: GOT2 Antibody (8B11) [NBP2-59428] - The Recombinant human GOT2 (50ng), Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: GOT2 Recombinant proteinLane 2.: Ramos cell lysateLane 3.: 293T cell lysateLane 4.: Liver tissue lysate

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 8B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GOT2 Antibody (8B11) - BSA Free Summary

• Description: Novus Biologicals Mouse GOT2 Antibody (8B11) - BSA Free (NBP2-59428) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-GOT2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human GOT2 (30-430aa) purified from E.coli.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1037
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30067865).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GOT2 Antibody (8B11) - BSA Free

• aspartate aminotransferase, mitochondrial
• EC 2.6.1
• EC 2.6.1.1
• FABP-1
• FABPpm
• Fatty acid-binding protein
• FLJ40994
• Glutamate oxaloacetate transaminase 2
• glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2)
• KAT4
• KATIV
• kynurenine aminotransferase IV
• mAspAT
• mitAAT
• Plasma membrane-associated fatty acid-binding protein
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOT2 Antibody (NBP2-59428)(1)

Publication using NBP2-59428

• Zabielski P, Daniluk J, Hady HR et al. The effect of high-fat diet and inhibition of ceramide production on insulin action in liver J. Cell. Physiol. 2018-08-01 [PMID: 30067865] (WB, Rat)

Bioinformatics

• Gene Symbol: GOT2