GOT2 Antibody (3E9)

Images

 
Western Blot: GOT2 Antibody (3E9) [NBP1-47469] - Analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
Immunohistochemistry-Paraffin: GOT2 Antibody (3E9) [NBP1-47469] - Analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P
Clone
3E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GOT2 Antibody (3E9) Summary

Immunogen
Purified recombinant fragment of human GOT2 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GOT2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for GOT2 Antibody (3E9)

  • aspartate aminotransferase, mitochondrial
  • EC 2.6.1
  • EC 2.6.1.1
  • FABP-1
  • FABPpm
  • Fatty acid-binding protein
  • FLJ40994
  • Glutamate oxaloacetate transaminase 2
  • glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2)
  • KAT4
  • KATIV
  • kynurenine aminotransferase IV
  • mAspAT
  • mitAAT
  • Plasma membrane-associated fatty acid-binding protein
  • Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOT2
Entrez
Uniprot