GOSR1 Antibody - BSA Free

Western Blot: GOSR1 Antibody - BSA Free [NBP3-46171] - Human liver tissue were subjected to SDS PAGE followed by western blot with (GOSR1 antibody) at dilution of 1:500

Immunohistochemistry: GOSR1 Antibody - BSA Free [NBP3-46171] - Immunohistochemistry of paraffin-embedded human stomach cancer using (GOSR1 antibody) at dilution of 1:50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, NULL
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GOSR1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GOSR1 Antibody - BSA Free (NBP3-46171) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: golgi SNAP receptor complex member 1. (Uniprot# O95249)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOSR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Serum FC
  • Western Blot 1:500-1:2000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GOSR1 Antibody - BSA Free

• golgi integral membrane protein 2
• golgi SNAP receptor complex member 1,28 kDa cis-Golgi SNARE p28
• Golgi SNARE 28 kDa
• GOLIM2
• GOS28cis-golgi SNARE
• GOS-28GOS28/P28
• GOSR1
• GS28
• GS2828 kDa Golgi SNARE protein
• p28

Background

In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum (ER) and delivers them after covalent modification to their destination in the cell. Although little is known about membrane-tubule-mediated transport, the molecular mechanism for vesicle-mediated transport is quite well understood, occurring through docking of SNAREs on the vesicle with those on the target membrane. (1). Transport of proteins along the exocytotic pathway is primarily achieved by vesicular intermediates. Two proteins, Golgi SNAREs of 27 kDa, GS27, and of 28 kDa, GS28 are important trafficking membrane proteins between the endoplasmic reticulum and the Golgi and between Golgi subcompartments (2). In vitro, GOS-28, A Golgi SNARE of 28 kD, is efficiently packaged into Golgi-derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOSR1