Goosecoid Overexpression Lysate

Images

 
Western Blot: GSC Overexpression Lysate (Adult Normal) [NBL1-11357] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Goosecoid Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209586 and based on accession number NM_173849. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens goosecoid homeobox (GSC), mRNA.
Gene
GSC

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Goosecoid Overexpression Lysate

  • goosecoid homeobox
  • Goosecoid
  • GSC
  • homeobox protein goosecoid

Background

This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-207
Species: Hu, Mu, Ce
Applications: WB, IHC, IHC-P, IP
NBP1-76963
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
AF1034
Species: Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-46076
Species: Hu
Applications: WB, IHC
H00007357-M03
Species: Hu
Applications: WB, ELISA
H00002729-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP1-91612
Species: Mu
Applications: WB
AF2085
Species: Hu, Mu
Applications: WB, IHC, ChIP, ICC
NB120-16518
Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-01926
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF2400
Species: Hu
Applications: WB, IHC, ChIP, ICC
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Goosecoid Lysate (NBL1-11357) (0)

There are no publications for Goosecoid Lysate (NBL1-11357).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Goosecoid Lysate (NBL1-11357) (0)

There are no reviews for Goosecoid Lysate (NBL1-11357). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Goosecoid Lysate (NBL1-11357) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Goosecoid Products

Bioinformatics Tool for Goosecoid Lysate (NBL1-11357)

Discover related pathways, diseases and genes to Goosecoid Lysate (NBL1-11357). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Goosecoid Lysate (NBL1-11357)

Discover more about diseases related to Goosecoid Lysate (NBL1-11357).
 

Pathways for Goosecoid Lysate (NBL1-11357)

View related products by pathway.

PTMs for Goosecoid Lysate (NBL1-11357)

Learn more about PTMs related to Goosecoid Lysate (NBL1-11357).

Blogs on Goosecoid

There are no specific blogs for Goosecoid, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Goosecoid Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol GSC