GOLPH4 Antibody (PSH02-98)

Immunohistochemistry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32399) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling GOLPH4 with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32399, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunocytochemistry/ Immunofluorescence: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunocytochemistry analysis of HeLa cells labeling GOLPH4 with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Flow cytometric analysis of HeLa cells labeling GOLPH4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32399, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Western blot analysis of GOLPH4 on different lysates with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: U-2 OS cell lysate Lane 4: LO2 cell lysate Lane 5: HUVEC cell lysate Lane 6: SiHa cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 82 kDa Observed band size: 130/82 kDa Exposure time: Lane 1-6 (left): 1 minute 2 seconds; Lane 1-6 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: PSH02-98
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GOLPH4 Antibody (PSH02-98) Summary

• Description: Novus Biologicals Rabbit GOLPH4 Antibody (PSH02-98) (NBP3-32399) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within human GOLPH4 aa 1-696 / 696. (Uniprot: O00461)
• Localization: Golgi apparatus > Golgi stack membrane. Endosome membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GOLIM4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:1500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:2000-1:10000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GOLPH4 Antibody (PSH02-98)

• GIMPc
• GIMPCcis
• golgi integral membrane protein 4
• Golgi phosphoprotein 4130 kDa golgi-localized phosphoprotein
• Golgi phosphoprotein of 130 kDa
• GPP130Golgi-localized phosphoprotein of 130 kDa

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOLIM4