GOLPH4 Antibodies
We offer GOLPH4 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each GOLPH4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GOLPH4 Antibodies can be used in a variety of model species: Human. Use the list below to choose the GOLPH4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GOLPH4 polyclonal antibodies and browse our GOLPH4 monoclonal antibody catalog.
Alternate Names for GOLPH4 Antibodies
anti-GOLPH4 antibody, anti-GOLIM4 antibody, anti-GIMPc antibody, anti-GIMPCcis antibody, anti-golgi integral membrane protein 4 antibody, anti-Golgi phosphoprotein 4130 kDa golgi-localized phosphoprotein antibody, anti-Golgi phosphoprotein of 130 kDa antibody, anti-GPP130Golgi-localized phosphoprotein of 130 kDa antibody
8 Results for "golph4" in Primary Antibodies
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91954] - Analysis in human small intestine and skin tissues using NBP1-91954 antibody. Corresponding GOLIM4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91954-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91954] - Analysis in human small intestine and skin tissues using NBP1-91954 antibody. Corresponding GOLIM4 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91954] - Staining of human cerebral cortex, liver, skin and small intestine using Anti-GOLIM4 antibody NBP1-91954 (A) shows similar protein distribution across tissues to independent antibody NBP1-91955 (B).](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91954-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: GOLPH4 Antibody [NBP1-91954] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91954-img0010.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91954] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91954-img0018.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91954] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91954-img0012.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91954] - Staining of human small intestine shows strong positivity in Golgi apparatus in glandular cells.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91954-img0016.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91954] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons and glial cells.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91954-img0017.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91954] - Staining of human skin shows moderate granular cytoplasmic positivity in a small subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91954-img0019.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0013.jpg "Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human liver shows strong cytoplasmic positivity with granular pattern in hepatocytes.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0002.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0003.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0004.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0007.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human liver.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human cerebral cortex, liver, skin and small intestine using Anti-GOLIM4 antibody NBP1-91955 (A) shows similar protein distribution across tissues to independent antibody NBP1-91954 (B).](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Analysis in human small intestine and skin tissues using NBP1-91955 antibody. Corresponding GOLIM4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0010.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human small intestine shows strong positivity in Golgi apparatus in glandular cells.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0011.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons and glial cells.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0012.jpg)
![Immunohistochemistry-Paraffin: GOLPH4 Antibody [NBP1-91955] - Staining of human skin shows moderate granular cytoplasmic positivity in a small subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Immunohistochemistry-Paraffin-NBP1-91955-img0014.jpg)
![Immunohistochemistry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32399) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-29820241492629.jpg "Immunohistochemistry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32399) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling GOLPH4 with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32399, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-298202414161893.jpg)
![Immunocytochemistry/ Immunofluorescence: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunocytochemistry analysis of HeLa cells labeling GOLPH4 with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-29202410334057.jpg)
![Flow Cytometry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Flow cytometric analysis of HeLa cells labeling GOLPH4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32399, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-29202416372195.jpg)
![Western Blot: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Western blot analysis of GOLPH4 on different lysates with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: U-2 OS cell lysate Lane 4: LO2 cell lysate Lane 5: HUVEC cell lysate Lane 6: SiHa cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 82 kDa Observed band size: 130/82 kDa Exposure time: Lane 1-6 (left): 1 minute 2 seconds; Lane 1-6 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-3920248485014.jpg)
![Western Blot: GOLPH4 Antibody (5E11) [H00027333-M01] - GOLPH4 monoclonal antibody (M01), clone 5E11 Analysis of GOLPH4 expression in HeLa.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-5E11-Western-Blot-H00027333-M01-img0007.jpg "Western Blot: GOLPH4 Antibody (5E11) [H00027333-M01] - GOLPH4 monoclonal antibody (M01), clone 5E11 Analysis of GOLPH4 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: GOLPH4 Antibody (5E11) [H00027333-M01] - Analysis of monoclonal antibody to GOLPH4 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-5E11-Immunocytochemistry-Immunofluorescence-H00027333-M01-img0008.jpg)
![Sandwich ELISA: GOLPH4 Antibody (5E11) [H00027333-M01] - Detection limit for recombinant GST tagged GOLPH4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-5E11-Sandwich-ELISA-H00027333-M01-img0006.jpg)
![Western Blot: GOLPH4 Antibody (SR1126) [NBP3-22031] - Western blot analysis using NBP3-22031 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22031_rabbit-golph4-mab-sr1126-57202316582144.jpg "Western Blot: GOLPH4 Antibody (SR1126) [NBP3-22031] - Western blot analysis using NBP3-22031 in HepG2 cell lysate.")
![Western Blot: GOLPH4 Antibody [NBP2-87517] - Host: Rabbit. Target Name: GOLIM4. Sample Tissue: Human COLO205 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Western-Blot-NBP2-87517-img0001.jpg "Western Blot: GOLPH4 Antibody [NBP2-87517] - Host: Rabbit. Target Name: GOLIM4. Sample Tissue: Human COLO205 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: GOLPH4 Antibody [NBP2-87516] - Host: Rabbit. Target Name: GOLIM4. Sample Tissue: Human U937 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/GOLPH4-Antibody-Western-Blot-NBP2-87516-img0001.jpg "Western Blot: GOLPH4 Antibody [NBP2-87516] - Host: Rabbit. Target Name: GOLIM4. Sample Tissue: Human U937 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: GOLPH4 Antibody (10J7E6) [NBP3-15917] - Western blot analysis of extracts of various cell lines, using GOLPH4 antibody (NBP3-15917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/GOLPH4-Antibody-10J7E6-Western-Blot-NBP3-15917-img0001.jpg "Western Blot: GOLPH4 Antibody (10J7E6) [NBP3-15917] - Western blot analysis of extracts of various cell lines, using GOLPH4 antibody (NBP3-15917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")