GOLM1 Overexpression Lysate (Native)

Images

 
Western Blot: GOLM1 Overexpression Lysate (Adult Normal) [NBL1-11198] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GOLM1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GOLM1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200086 and based on accession number NM_177937. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens golgi membrane protein 1 (GOLM1), transcript variant 2, mRNA.
Gene
GOLM1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GOLM1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GOLM1 Overexpression Lysate (Native)

  • bA379P1.3
  • FLJ23608
  • golgi membrane protein 1
  • Golgi membrane protein GP73
  • Golgi phosphoprotein 2C9orf155
  • golgi protein, 73-kD
  • GOLPH2 FLJ22634
  • GP73 chromosome 9 open reading frame 155
  • PSEC0257

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1368
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICC, ICFlow, KO
NBP1-81804
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP1-83910
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13980
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-28884
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-91954
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-26642
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-33090
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
AF4036
Species: Hu
Applications: WB, Simple Western
H00005324-M02
Species: Hu
Applications: WB, ELISA, IHC
NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24482
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB
NB100-355
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Xp
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
AF2747
Species: Mu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP1-58268
Species: Hu
Applications: WB, IHC, IHC-P
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-30003
Species: Hu
Applications: WB, ICC/IF, IHC-P

Publications for GOLM1 Lysate (NBL1-11198) (0)

There are no publications for GOLM1 Lysate (NBL1-11198).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GOLM1 Lysate (NBL1-11198) (0)

There are no reviews for GOLM1 Lysate (NBL1-11198). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GOLM1 Lysate (NBL1-11198) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GOLM1 Products

Bioinformatics Tool for GOLM1 Lysate (NBL1-11198)

Discover related pathways, diseases and genes to GOLM1 Lysate (NBL1-11198). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GOLM1 Lysate (NBL1-11198)

Discover more about diseases related to GOLM1 Lysate (NBL1-11198).
 

Pathways for GOLM1 Lysate (NBL1-11198)

View related products by pathway.

PTMs for GOLM1 Lysate (NBL1-11198)

Learn more about PTMs related to GOLM1 Lysate (NBL1-11198).
 

Research Areas for GOLM1 Lysate (NBL1-11198)

Find related products by research area.

Blogs on GOLM1.

6 Fictional Character Protein Names
Check out our list of some of the funniest fictional character protein names including GOLM1, SMURF1, HOMER1, Hunchback, Lilliputian, Pokemon, Tinman Paralog, and Draculin. What are your favorite or funniest protein names? Share them in the comments;...  Read full blog post.

Customers Who Bought This Also Bought

GOLM1 Antibody
NBP1-50627

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GOLM1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GOLM1