GOLM1 Antibody (5B10) - Azide and BSA Free

Western Blot: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of GOLM1 expression in transfected 293T cell line by GOLM1 monoclonal antibody (M06), clone 5B10. Lane 1: GOLM1 transfected lysatE (45.3 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of monoclonal antibody to GOLM1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of monoclonal antibody to GOLM1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml

ELISA: GOLM1 Antibody (5B10) [H00051280-M06] - Detection limit for recombinant GST tagged GOLPH2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 5B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GOLM1 Antibody (5B10) - Azide and BSA Free Summary

• Immunogen: GOLM1 (NP_057632, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL
• Specificity: GOLPH2 - golgi phosphoprotein 2 (5B10)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOLM1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GOLM1 Antibody (5B10) - Azide and BSA Free

• bA379P1.3
• FLJ23608
• golgi membrane protein 1
• Golgi membrane protein GP73
• Golgi phosphoprotein 2C9orf155
• golgi protein, 73-kD
• GOLPH2 FLJ22634
• GP73 chromosome 9 open reading frame 155
• GP73
• PSEC0257

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOLM1 Antibody (H00051280-M06)(6)

Publications using H00051280-M06

• Liewen H, Markuly N, Laubli H et al. Therapeutic Targeting of Golgi Phosphoprotein 2 (GOLPH2) with Armed Antibodies: A Preclinical Study of Anti-GOLPH2 Antibody Drug Conjugates in Lung and Colorectal Cancer Models of Patient Derived Xenografts (PDX). Target Oncol. 2019-10-15 [PMID: 31541350]
• Hirst J, Itzhak D, Antrobus R et al. Role of the AP-5 adaptor protein complex in late endosome-to-Golgi retrieval. PLoS Biol. 2018-01-15 [PMID: 29381698]
• Braun M, Menon R, Nikolov P et al. The HOPE fixation technique - a promising alternative to common prostate cancer biobanking approaches. BMC Cancer. 2011-12-07 [PMID: 22151117]
• Scheble VJ, Braun M, Wilbertz T et al. ERG rearrangement in small cell prostatic and lung cancer. Histopathology. 2010-01-10 [PMID: 20636794]
• Kristiansen G, Fritzsche FR, Wassermann K et al. GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics. Br J Cancer. 2008-09-16 [PMID: 18781151]
• Wei S, Dunn TA, Isaacs WB et al. GOLPH2 and MYO6: putative prostate cancer markers localized to the Golgi apparatus. Prostate. 2008-09-15 [PMID: 18543251]

Bioinformatics

• Gene Symbol: GOLM1
• OMIM:
  • 606804(Human)