GM-CSFR alpha Antibody (31916) [Allophycocyanin]

• Applications: Flow
• Clone: 31916
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

GM-CSFR alpha Antibody (31916) [Allophycocyanin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GM-CSF R alpha
  Extracellular domain
• Specificity: Detects human GM-CSF R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) M‑CSF R or rhGM‑CSF R beta ( beta _c) is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF2RA
• Purity Statement: Protein A or G purified from ascites

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Granulocyte Macrophage Colony Stimulating Factor Receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1‑4). Mature human GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 26 aa transmembrane segment, and a 54 aa cytoplasmic domain (5). Within the ECD, human GM-CSF R alpha shares approximately 33% aa sequence identity with mouse and rat GM-CSF R alpha . Alternate splicing of human GM-CSF R alpha generates several additional isoforms that lack the cytoplasmic and/or transmembrane regions. Soluble forms of the receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non-hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta _c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta _c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9‑11). The shared usage of beta _c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
2. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
5. Gearing, D.P. et al. (1989) EMBO J. 8:3667.
6. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
7. Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
8. Chiba, S. et al. (1990) Cell Regul. 1:327.
9. Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
10. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
11. Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF2RA