Glypican 2 Antibody (300712) [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB
• Clone: 300712
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

Glypican 2 Antibody (300712) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Glypican 2
  Lys27-Leu554
  Accession # Q8BKV1
• Specificity: Detects mouse Glypican 2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Glypican 2, 3, 6, or recombinant mouse Glypican 5 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 2 Antibody (300712) [Alexa Fluor® 350]

• 2410016G05Rik
• Cerebroglycan
• Glypican 2
• GPC2

Background

The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1‑GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35‑63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17‑25% sequence similarity (1‑4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine‑GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.