Glyoxalase I Antibody (266)

Images

 
Genetic Strategies: Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted ...read more
Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ...read more
Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian ...read more
Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract D. 30 ug A375 whole cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clone
266
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Glyoxalase I Antibody (266) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.
Predicted Species
Mouse (92%), Rat (91%), Rhesus Macaque (97%), Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GLO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Glyoxalase I Antibody (266)

  • Aldoketomutase
  • EC 4.4.1.5
  • GLO1
  • GLOD1
  • Glx I
  • GLYI
  • glyoxalase domain containing 1
  • Glyoxalase I
  • glyoxalase Ialdoketomutase
  • Ketone-aldehyde mutase
  • lactoyl glutathione lyase
  • lactoylglutathione lyase
  • Methylglyoxalase
  • S-D-lactoylglutathione methylglyoxal lyase

Background

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glyoxalase I Antibody (NBP2-43618) (0)

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Product General Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Glyoxalase I Antibody (NBP2-43618)

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Bioinformatics

Gene Symbol GLO1