Glyoxalase I Antibody (266)

Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [266] diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glyoxalase I stained by Glyoxalase I Antibody (266) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I Antibody (266) diluted at 1:200.

Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract D. 30 ug A375 whole cell lysate/extract 12 % SDS-PAGE Glyoxalase I antibody [266] dilution: 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF, IHC-P
• Clone: 266
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glyoxalase I Antibody (266) Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.
• Predicted Species: Mouse (92%), Rat (91%), Rhesus Macaque (97%), Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLO1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Glyoxalase I Antibody (266)

• Aldoketomutase
• EC 4.4.1.5
• GLO1
• GLOD1
• Glx I
• GLYI
• glyoxalase domain containing 1
• Glyoxalase I
• glyoxalase Ialdoketomutase
• Ketone-aldehyde mutase
• lactoyl glutathione lyase
• lactoylglutathione lyase
• Methylglyoxalase
• S-D-lactoylglutathione methylglyoxal lyase

Background

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLO1