Glycophorin A + B Antibody (E3)

Product Discontinued

Glycophorin A + B Antibody (E3) Summary

• Immunogen: human thymus
• Specificity: Binds to red blood cell glycophorins A & B (glycophorin alpha and Delta)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GYPA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: Use at a dilution of 1/6400. Flow Cytometry Use at a dilution of 1/400 (10ul per million cells). WB: Use at a dilution of 1/400. Agglutination: Use at a dilution of 1/1000Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Glycophorin A + B Antibody (E3)

• CD235a antigen
• glycophorin A (includes MN blood group)
• glycophorin A (MNS blood group)
• glycophorin Erik
• glycophorin MiI
• glycophorin MiIII
• glycophorin MiV
• glycophorin MiX
• glycophorin SAT
• glycophorin Sta type C
• glycophorin-A
• GPA
• GPErik
• GpMiIII
• GPSAT
• HGpMiIII
• HGpMiV
• HGpMiX
• HGpMiXI
• HGpSta(C)
• Mi.V glycoprotein (24 AA)
• MN sialoglycoprotein
• MNS
• PAS-2
• recombinant glycophorin A-B Miltenberger-DR

Background

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glycophorin A + B Antibody (NB120-6396)(1)

Publication using NB120-6396

• Cané L, Saffioti NA, Genetet S et al. Alpha hemolysin of E. coli induces hemolysis of human erythrocytes independently of toxin interaction with membrane proteins Biochimie 2023-10-13 [PMID: 37820991] (Human)

Bioinformatics

• Gene Symbol: GYPA
• Entrez:
  • 2993(Human)