Glycophorin A Antibody (HIR2) [PE]

Product Discontinued

Glycophorin A Antibody (HIR2) [PE] Summary

• Immunogen: Raji cells and spleen cells fused with NS1 cells
• Specificity: The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GYPA
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for Glycophorin A Antibody (HIR2) [PE]

• CD235a antigen
• CD235a
• glycophorin A (includes MN blood group)
• glycophorin A (MNS blood group)
• Glycophorin A
• glycophorin Erik
• glycophorin MiI
• glycophorin MiIII
• glycophorin MiV
• glycophorin MiX
• glycophorin SAT
• glycophorin Sta type C
• glycophorin-A
• GPA
• GPErik
• GpMiIII
• GPSAT
• GYPA
• HGpMiIII
• HGpMiV
• HGpMiX
• HGpMiXI
• HGpSta(C)
• Mi.V glycoprotein (24 AA)
• MN sialoglycoprotein
• MNS
• PAS-2
• recombinant glycophorin A-B Miltenberger-DR

Background

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glycophorin A Antibody (NB500-587)(1)

Publication using NB500-587

• Belizaire RM, Prakash PS, Richter JR, Robinson BR, Edwards MJ, Caldwell CC, Lentsch AB, Pritts TA. Microparticles from Stored Red Blood Cells Activate Neutrophils and Cause Lung Injury after Hemorrhage and Resuscitation. J Am Coll Surg. 2012-02-16 [PMID: 22342784]

Bioinformatics

• Gene Symbol: GYPA