Glycine Receptor Alpha 1 Antibody

Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa alpha 1- and alpha 2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.

Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord showing specific immunolabeling of the ~48k a1- and a2-subunits of the glycine receptor. The labeling was absent from a Rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.

Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Western blot of 10 ug of rat spinal cord. The antibody is specific for the ~48 kDa a1- and a2-subunitsof the glycine receptor.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-Fr, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Glycine Receptor Alpha 1 Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P07727
• Localization: Cell membrane
• Specificity: Specific for the ~48 kDa alpha1- and alpha2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Does not recognize other glycine receptor subunits.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLRA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: Use in immunoprecipitation reported in scientific literature (PMID:31757808).
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Antigen Affinity-purified
• Reconstitution Instructions: Reconstitute with 50 uL PBS to desired concentration.

Alternate Names for Glycine Receptor Alpha 1 Antibody

• GLRA 1
• GLRA1
• Glycine receptor 48 kDa subunit
• Glycine receptor strychnine-binding subunit
• glycine receptor subunit alpha-1
• glycine receptor, alpha 1 (startle disease/hyperekplexia)
• glycine receptor, alpha 1
• HKPX1
• MGC138878
• MGC138879
• STHE

Background

Glycine is an important inhibitory neurotransmitter in the mammalian central nervous system, especially in the brainstem and spinal cord. It acts by binding to anion-conducting glycine receptors that belong to a superfamily of ligand-gated ion channels. Glycine receptors were first purified as strychnine binding sites in membrane fractions of adult spinal cord from rats (1). These strychnine-sensitive binding sites are different from the strychnine-insensitive binding sites found in the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Adult glycine receptors are heterodimers composed of two subunits of 48 kDa and 58 kDa, denoted as a1 and b subunits. Fetal glycine receptors are homomers composed of a2 subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glycine Receptor Alpha 1 Antibody (NB300-113)(3)

Publications using NB300-113

• Zou G, Xia J, Han Q et al. The synthetic cannabinoid dehydroxylcannabidiol restores the function of a major GABAA receptor isoform in a cell model of hyperekplexia J. Biol. Chem. 2019-11-22 [PMID: 31757808] (IP, Human)
• Zou G, Chen Q et al. Human Hyperekplexic Mutations in Glycine Receptors Disinhibit the Brainstem by Hijacking GABAA Receptors. iScience 2019-08-13 [PMID: 31450193] (WB, Human)
• Lu J, Fan S, Zou G, Hou Y. Involvement of glycine receptor a1 subunits in cannabinoid-induced analgesia. Neuropharmacology. 2018-02-01 [PMID: 29407767] (WB, Mouse)

FAQs for Glycine Receptor Alpha 1 Antibody (NB300-113). (Showing 1 - 1 of 1 FAQs).

1. Can you please let me know if you have antibodies against the following? For nAChR alpha 7 receptor of human origin - between amino acids 321- 465 For Glycine alpha1 receptor of human origin - between amino acids 339-413 For 5-HT 3A receptor of mouse origin - between amino acids 338-452
   • We do not routinely epitope map our antibodies, and do not typically know where the antigen/antibody cites are. On occasion, customers will provide feedback about the epitopes, but this is not the norm. Unfortunately, we do not have any antibodies for nAChR alpha 7 receptor. For alpha 1 Glycine Receptor, we have two antibodies that target the N-terminus, NB300-113 and NBP1-49174, but I do not have more specific information about the epitope. For 5HT3A, we do have one antibody that may suit your needs, NB100-56351.

Bioinformatics

• Gene Symbol: GLRA1
• Entrez:
  • 2741(Human)
  • 14654(Mouse)
  • 25674(Rat)
• Uniprot:
  • P07727(Rat)