Novus Biologicals products are now on bio-techne.com

Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

Images

 
Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa alpha 1- and alpha 2-subunits of the glycine receptor. Immunolabeling was absent ...read more
Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord showing specific immunolabeling of the ~48k a1- and a2-subunits of the glycine receptor. The labeling was absent from a Rat hippocampal ...read more
Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Western blot of 10 ug of rat spinal cord. The antibody is specific for the ~48 kDa a1- and a2-subunitsof the glycine receptor.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Glycine Receptor Alpha 1 Antibody - Azide and BSA Free Summary

Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P07727
Localization
Cell membrane
Specificity
Specific for the ~48 kDa alpha1- and alpha2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Does not recognize other glycine receptor subunits.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLRA1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID:31757808).
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB300-113 in the following applications:

  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute with 50 uL PBS to desired concentration.

Alternate Names for Glycine Receptor Alpha 1 Antibody - Azide and BSA Free

  • GLRA 1
  • GLRA1
  • Glycine receptor 48 kDa subunit
  • Glycine receptor strychnine-binding subunit
  • glycine receptor subunit alpha-1
  • glycine receptor, alpha 1 (startle disease/hyperekplexia)
  • glycine receptor, alpha 1
  • HKPX1
  • MGC138878
  • MGC138879
  • STHE

Background

Glycine is an important inhibitory neurotransmitter in the mammalian central nervous system, especially in the brainstem and spinal cord. It acts by binding to anion-conducting glycine receptors that belong to a superfamily of ligand-gated ion channels. Glycine receptors were first purified as strychnine binding sites in membrane fractions of adult spinal cord from rats (1). These strychnine-sensitive binding sites are different from the strychnine-insensitive binding sites found in the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Adult glycine receptors are heterodimers composed of two subunits of 48 kDa and 58 kDa, denoted as a1 and b subunits. Fetal glycine receptors are homomers composed of a2 subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-61774
Species: Hu
Applications: ELISA, Flow, ICC/IF, WB
NBP2-86653
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-03449
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP3-12251
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP1-85533
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24716
Species: Hu, Mu, Pm
Applications: ICC/IF, IHC, IHC-P, WB
MAB7848
Species: Hu
Applications: IHC, WB
H00008001-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-42048
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP3-03685
Species: Hu, Mu, Rt
Applications: WB
NBP2-75091
Species: Hu, Rt
Applications: ICC/IF, IHC-P, PEP-ELISA, WB
NBP1-87466
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
AF2086
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
NBP2-25162
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, PLA, WB
NB300-191
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IP, KO, WB

Publications for Glycine Receptor Alpha 1 Antibody (NB300-113)(4)

Reviews for Glycine Receptor Alpha 1 Antibody (NB300-113) (0)

There are no reviews for Glycine Receptor Alpha 1 Antibody (NB300-113). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Glycine Receptor Alpha 1 Antibody (NB300-113). (Showing 1 - 1 of 1 FAQs).

  1. Can you please let me know if you have antibodies against the following? For nAChR alpha 7 receptor of human origin - between amino acids 321- 465 For Glycine alpha1 receptor of human origin - between amino acids 339-413 For 5-HT 3A receptor of mouse origin - between amino acids 338-452
    • We do not routinely epitope map our antibodies, and do not typically know where the antigen/antibody cites are. On occasion, customers will provide feedback about the epitopes, but this is not the norm. Unfortunately, we do not have any antibodies for nAChR alpha 7 receptor. For alpha 1 Glycine Receptor, we have two antibodies that target the N-terminus, NB300-113 and NBP1-49174, but I do not have more specific information about the epitope. For 5HT3A, we do have one antibody that may suit your needs, NB100-56351.

Secondary Antibodies

 

Isotype Controls

Additional Glycine Receptor Alpha 1 Products

Research Areas for Glycine Receptor Alpha 1 Antibody (NB300-113)

Find related products by research area.

Blogs on Glycine Receptor Alpha 1

There are no specific blogs for Glycine Receptor Alpha 1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Glycine Receptor Alpha 1 Antibody - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol GLRA1
Entrez
Uniprot